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3r5k
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| - | [[ | + | ==A designed redox-controlled caspase-7== |
| + | <StructureSection load='3r5k' size='340' side='right' caption='[[3r5k]], [[Resolution|resolution]] 2.86Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3r5k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R5K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R5K FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1f1j|1f1j]], [[1i4o|1i4o]], [[1k86|1k86]], [[1k88|1k88]], [[1gqf|1gqf]], [[1kmc|1kmc]], [[1i51|1i51]], [[1shj|1shj]], [[1shl|1shl]], [[2ql5|2ql5]], [[2ql7|2ql7]], [[2ql9|2ql9]], [[2qlb|2qlb]], [[2qlf|2qlf]], [[2qlj|2qlj]], [[3edr|3edr]], [[3h1p|3h1p]], [[3ibc|3ibc]], [[3ibf|3ibf]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CASP7, MCH3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Caspase-7 Caspase-7], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.60 3.4.22.60] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r5k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r5k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r5k RCSB], [http://www.ebi.ac.uk/pdbsum/3r5k PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Caspases are a powerful class of cysteine proteases. Introduction of activated caspases in healthy or cancerous cells results in induction of apoptotic cell death. We have designed and characterized a version of caspase-7 that can be inactivated under oxidizing conditions like those found extracellularly, then reactivated under reducing, intracellular conditions. This version of caspase-7 is allosterically inactivated when two of the substrate-binding loops are locked together via an engineered disulfide. When this disulfide is reduced, the protein regains full function. The inactive loop-locked version of caspase-7 can be readily observed by immunoblotting and by mass spectrometry. The reduced and reactivated form of the enzyme observed crystallographically is the first caspase-7 structure in which the substrate-binding groove is properly ordered even in the absence of an active-site ligand. In the reactivated structure, the catalytic dyad cysteine-histidine are positioned 3.5 A apart in an orientation that is capable of supporting catalysis. This redox-controlled version of caspase-7 is particularly well-suited for targeted cell death in concert with redox-triggered delivery vehicles. | ||
| - | + | A designed redox-controlled caspase.,Witkowski WA, Hardy JA Protein Sci. 2011 Jun 14. doi: 10.1002/pro.673. PMID:21674661<ref>PMID:21674661</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | ||
| - | + | ||
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| - | + | ||
==See Also== | ==See Also== | ||
*[[Caspase|Caspase]] | *[[Caspase|Caspase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Caspase-7]] | [[Category: Caspase-7]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Hardy, J A | + | [[Category: Hardy, J A]] |
| - | [[Category: Witkowski, W A | + | [[Category: Witkowski, W A]] |
[[Category: Apoptosis]] | [[Category: Apoptosis]] | ||
[[Category: Caspase]] | [[Category: Caspase]] | ||
Revision as of 12:20, 9 December 2014
A designed redox-controlled caspase-7
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