3hzq

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[[Image:3hzq.png|left|200px]]
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==Structure of a tetrameric MscL in an expanded intermediate state==
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<StructureSection load='3hzq' size='340' side='right' caption='[[3hzq]], [[Resolution|resolution]] 3.82&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3hzq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mw2 Staphylococcus aureus subsp. aureus mw2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HZQ FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mscL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196620 Staphylococcus aureus subsp. aureus MW2])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hzq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hzq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hzq RCSB], [http://www.ebi.ac.uk/pdbsum/3hzq PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hz/3hzq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The ability of cells to sense and respond to mechanical force underlies diverse processes such as touch and hearing in animals, gravitropism in plants, and bacterial osmoregulation. In bacteria, mechanosensation is mediated by the mechanosensitive channels of large (MscL), small (MscS), potassium-dependent (MscK) and mini (MscM) conductances. These channels act as 'emergency relief valves' protecting bacteria from lysis upon acute osmotic down-shock. Among them, MscL has been intensively studied since the original identification and characterization 15 years ago. MscL is reversibly and directly gated by changes in membrane tension. In the open state, MscL forms a non-selective 3 nS conductance channel which gates at tensions close to the lytic limit of the bacterial membrane. An earlier crystal structure at 3.5 A resolution of a pentameric MscL from Mycobacterium tuberculosis represents a closed-state or non-conducting conformation. MscL has a complex gating behaviour; it exhibits several intermediates between the closed and open states, including one putative non-conductive expanded state and at least three sub-conducting states. Although our understanding of the closed and open states of MscL has been increasing, little is known about the structures of the intermediate states despite their importance in elucidating the complete gating process of MscL. Here we present the crystal structure of a carboxy-terminal truncation mutant (Delta95-120) of MscL from Staphylococcus aureus (SaMscL(CDelta26)) at 3.8 A resolution. Notably, SaMscL(CDelta26) forms a tetrameric channel with both transmembrane helices tilted away from the membrane normal at angles close to that inferred for the open state, probably corresponding to a non-conductive but partially expanded intermediate state.
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{{STRUCTURE_3hzq| PDB=3hzq | SCENE= }}
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Structure of a tetrameric MscL in an expanded intermediate state.,Liu Z, Gandhi CS, Rees DC Nature. 2009 Sep 3;461(7260):120-4. Epub 2009 Aug 23. PMID:19701184<ref>PMID:19701184</ref>
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===Structure of a tetrameric MscL in an expanded intermediate state===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19701184}}
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==About this Structure==
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[[3hzq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mw2 Staphylococcus aureus subsp. aureus mw2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HZQ OCA].
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==See Also==
==See Also==
*[[Ion channels|Ion channels]]
*[[Ion channels|Ion channels]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019701184</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Staphylococcus aureus subsp. aureus mw2]]
[[Category: Staphylococcus aureus subsp. aureus mw2]]
[[Category: Gandhi, C S.]]
[[Category: Gandhi, C S.]]

Revision as of 13:21, 29 September 2014

Structure of a tetrameric MscL in an expanded intermediate state

3hzq, resolution 3.82Å

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