3pel
From Proteopedia
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| - | [[ | + | ==Structure of Greyhound Hemoglobin: Origin of High Oxygen Affinity== |
| + | <StructureSection load='3pel' size='340' side='right' caption='[[3pel]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3pel]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3n48 3n48]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PEL FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pel OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pel RCSB], [http://www.ebi.ac.uk/pdbsum/3pel PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | This study presents the crystal structure of Greyhound hemoglobin (GrHb) determined to 1.9 A resolution. GrHb was found to crystallize with an alpha(1)beta(1) dimer in the asymmetric unit and belongs to the R2 state. Oxygen-affinity measurements combined with the fact that GrHb crystallizes in the R2 state despite the high-salt conditions used for crystallization strongly indicate that GrHb can serve as a model high-oxygen-affinity hemoglobin (Hb) for higher mammals, especially humans. Structural analysis of GrHb and its comparison with the R2-state of human Hb revealed several regions that can potentially contribute to the high oxygen affinity of GrHb and serve to rationalize the additional stability of the R2-state of GrHb. A previously well studied hydrophobic cluster of bar-headed goose Hb near alpha119 was also incorporated in the comparison between GrHb and human Hb. Finally, a structural comparison with generic dog Hb and maned wolf Hb was conducted, revealing that in contrast to GrHb these structures belong to the R state of Hb and raising the intriguing possibility of an additional allosteric factor co-purifying with GrHb that can modulate its quaternary structure. | ||
| - | + | Structure of Greyhound hemoglobin: origin of high oxygen affinity.,Bhatt VS, Zaldivar-Lopez S, Harris DR, Couto CG, Wang PG, Palmer AF Acta Crystallogr D Biol Crystallogr. 2011 May;67(Pt 5):395-402. Epub 2011, Apr 13. PMID:21543841<ref>PMID:21543841</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
| - | *[[Hemoglobin|Hemoglobin]] | + | *[[Hemoglobin 3D structures|Hemoglobin 3D structures]] |
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Canis lupus familiaris]] | [[Category: Canis lupus familiaris]] | ||
| - | [[Category: Bhatt, V S | + | [[Category: Bhatt, V S]] |
| - | [[Category: Couto, C G | + | [[Category: Couto, C G]] |
| - | [[Category: Harris, D R | + | [[Category: Harris, D R]] |
| - | [[Category: Palmer, A F | + | [[Category: Palmer, A F]] |
| - | [[Category: Wang, P G | + | [[Category: Wang, P G]] |
| - | [[Category: Zaldivar-Lopez, S | + | [[Category: Zaldivar-Lopez, S]] |
[[Category: Globin fold]] | [[Category: Globin fold]] | ||
[[Category: Heme binding]] | [[Category: Heme binding]] | ||
Revision as of 12:33, 9 December 2014
Structure of Greyhound Hemoglobin: Origin of High Oxygen Affinity
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