2enx

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[[Image:2enx.png|left|200px]]
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==Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution==
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<StructureSection load='2enx' size='340' side='right' caption='[[2enx]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2enx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_a909 Streptococcus agalactiae a909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ENX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ENX FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PN:IMIDODIPHOSPHORIC+ACID'>2PN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene><br>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ppaC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=205921 Streptococcus agalactiae A909])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2enx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2enx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2enx RCSB], [http://www.ebi.ac.uk/pdbsum/2enx PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/en/2enx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Streptococcus agalactiae, a prokaryote that causes infections in neonates and immunocompromised adults, has a serine/threonine protein kinase (STK) signalling cascade. The structure of one of the targets, a family II inorganic pyrophosphatase, has been solved by molecular replacement and refined at 2.80 A resolution to an R factor of 19.2% (R(free) = 26.7%). The two monomers in the asymmetric unit are related by a noncrystallographic twofold axis, but the biological dimer is formed by a crystallographic twofold. Each monomer contains the pyrophosphate analogue imidodiphosphate (PNP) and three metal ions per active site: two Mn(2+) ions in sites M1 and M2 and an Mg(2+) ion in site M3. The enzyme is in the closed conformation. Like other family II enzymes, the structure consists of two domains (residues 1-191 and 198-311), with the active site located between them. The conformation of Lys298 in the active site is different from those observed previously and it coordinates to the conserved DHH motif in a unique way. The structure suggests that Ser150, Ser194, Ser195 and Ser296 are the most likely targets for the Ser/Thr kinase and phosphatase because they are surface-accessible and either in the active site or in the hinge region between the two domains.
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{{STRUCTURE_2enx| PDB=2enx | SCENE= }}
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Structure of the Streptococcus agalactiae family II inorganic pyrophosphatase at 2.80 A resolution.,Rantanen MK, Lehtio L, Rajagopal L, Rubens CE, Goldman A Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):738-43. Epub 2007, May 15. PMID:17505113<ref>PMID:17505113</ref>
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===Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_17505113}}
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==About this Structure==
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[[2enx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae_a909 Streptococcus agalactiae a909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ENX OCA].
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==See Also==
==See Also==
*[[Inorganic pyrophosphatase|Inorganic pyrophosphatase]]
*[[Inorganic pyrophosphatase|Inorganic pyrophosphatase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:017505113</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Inorganic diphosphatase]]
[[Category: Inorganic diphosphatase]]
[[Category: Streptococcus agalactiae a909]]
[[Category: Streptococcus agalactiae a909]]

Revision as of 04:31, 29 September 2014

Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution

2enx, resolution 2.80Å

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