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3rlb
From Proteopedia
(Difference between revisions)
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| - | [[ | + | ==Crystal structure at 2.0 A of the S-component for thiamin from an ECF-type ABC transporter== |
| + | <StructureSection load='3rlb' size='340' side='right' caption='[[3rlb]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3rlb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._cremoris Lactococcus lactis subsp. cremoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RLB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RLB FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=VIB:3-(4-AMINO-2-METHYL-PYRIMIDIN-5-YLMETHYL)-5-(2-HYDROXY-ETHYL)-4-METHYL-THIAZOL-3-IUM'>VIB</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LLNZ_01755 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1359 Lactococcus lactis subsp. cremoris])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rlb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rlb RCSB], [http://www.ebi.ac.uk/pdbsum/3rlb PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Energy coupling factor (ECF) transporters are used for the uptake of vitamins in Prokarya. They consist of an integral membrane protein that confers substrate specificity (the S-component) and an energizing module that is related to ATP-binding cassette (ABC) transporters. S-components for different substrates often do not share detectable sequence similarity but interact with the same energizing module. Here we present the crystal structure of the thiamine-specific S-component ThiT from Lactococcus lactis at 2.0 A. Extensive protein-substrate interactions explain its high binding affinity for thiamine (K(d) ~10(-10) M). ThiT has a fold similar to that of the riboflavin-specific S-component RibU, with which it shares only 14% sequence identity. Two alanines in a conserved motif (AxxxA) located on the membrane-embedded surface of the S-components mediate the interaction with the energizing module. Based on these findings, we propose a general transport mechanism for ECF transporters. | ||
| - | + | The structural basis of modularity in ECF-type ABC transporters.,Erkens GB, Berntsson RP, Fulyani F, Majsnerowska M, Vujicic-Zagar A, Ter Beek J, Poolman B, Slotboom DJ Nat Struct Mol Biol. 2011 Jun 26;18(7):755-60. doi: 10.1038/nsmb.2073. PMID:21706007<ref>PMID:21706007</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[ABC transporter|ABC transporter]] | *[[ABC transporter|ABC transporter]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Lactococcus lactis subsp. cremoris]] | [[Category: Lactococcus lactis subsp. cremoris]] | ||
| - | [[Category: Beek, J ter | + | [[Category: Beek, J ter]] |
| - | [[Category: Berntsson, R P.A | + | [[Category: Berntsson, R P.A]] |
| - | [[Category: Erkens, G B | + | [[Category: Erkens, G B]] |
| - | [[Category: Fulyani, F | + | [[Category: Fulyani, F]] |
| - | [[Category: Majsnerowska, M | + | [[Category: Majsnerowska, M]] |
| - | [[Category: Poolman, B | + | [[Category: Poolman, B]] |
| - | [[Category: Slotboom, D J | + | [[Category: Slotboom, D J]] |
| - | [[Category: Vujicic-Zagar, A | + | [[Category: Vujicic-Zagar, A]] |
[[Category: Abc transporter]] | [[Category: Abc transporter]] | ||
[[Category: Ecf transporter]] | [[Category: Ecf transporter]] | ||
Revision as of 12:12, 9 December 2014
Crystal structure at 2.0 A of the S-component for thiamin from an ECF-type ABC transporter
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