1nat

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[[Image:1nat.gif|left|200px]]<br /><applet load="1nat" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1nat.gif|left|200px]]
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caption="1nat, resolution 2.45&Aring;" />
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'''CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS'''<br />
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{{Structure
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|PDB= 1nat |SIZE=350|CAPTION= <scene name='initialview01'>1nat</scene>, resolution 2.45&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE= SPO0F ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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}}
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'''CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1NAT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NAT OCA].
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1NAT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NAT OCA].
==Reference==
==Reference==
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A response regulatory protein with the site of phosphorylation blocked by an arginine interaction: crystal structure of Spo0F from Bacillus subtilis., Madhusudan M, Zapf J, Hoch JA, Whiteley JM, Xuong NH, Varughese KI, Biochemistry. 1997 Oct 21;36(42):12739-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9335530 9335530]
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A response regulatory protein with the site of phosphorylation blocked by an arginine interaction: crystal structure of Spo0F from Bacillus subtilis., Madhusudan M, Zapf J, Hoch JA, Whiteley JM, Xuong NH, Varughese KI, Biochemistry. 1997 Oct 21;36(42):12739-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9335530 9335530]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: two component system]]
[[Category: two component system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:04:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:54:21 2008''

Revision as of 10:54, 20 March 2008


PDB ID 1nat

Drag the structure with the mouse to rotate
, resolution 2.45Å
Gene: SPO0F (Bacillus subtilis)
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS


Overview

Spo0F is a secondary messenger in the "two-component" system controlling the sporulation of Bacillus subtilis. Spo0F, like the chemotaxis protein CheY, is a single-domain protein homologous to the N-terminal activator domain of the response regulators. We recently reported the crystal structure of a phosphatase-resistant mutant Y13S of Spo0F with Ca2+ bound in the active site. The crystal structure of wild-type Spo0F in the absence of a metal ion is presented here. A comparison of the two structures reveals that the cation induces significant changes in the active site. In the present wild-type structure, the carboxylate of Asp11 points away from the center of the active site, whereas when coordinated to the Ca2+, as in the earlier structure, it points toward the active site. In addition, Asp54, the site of phosphorylation, is blocked by a salt bridge interaction of an Arg side chain from a neighboring molecule. From fluorescence quenching studies with Spo0F Y13W, we found that only the amino acid Arg binds to Spo0F in a saturable manner (Kd = 15 mM). This observation suggests that a small molecule with a shape complementary to the active site and having a guanidinium group might inhibit phosphotransfer between response regulators and their cognate histidine kinases.

About this Structure

1NAT is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

A response regulatory protein with the site of phosphorylation blocked by an arginine interaction: crystal structure of Spo0F from Bacillus subtilis., Madhusudan M, Zapf J, Hoch JA, Whiteley JM, Xuong NH, Varughese KI, Biochemistry. 1997 Oct 21;36(42):12739-45. PMID:9335530

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