1ndl
From Proteopedia
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| - | [[Image:1ndl.jpg|left|200px]] | + | [[Image:1ndl.jpg|left|200px]] |
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| - | '''THE AWD NUCLEOTIDE DIPHOSPHATE KINASE FROM DROSOPHILA''' | + | {{Structure |
| + | |PDB= 1ndl |SIZE=350|CAPTION= <scene name='initialview01'>1ndl</scene>, resolution 2.4Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THE AWD NUCLEOTIDE DIPHOSPHATE KINASE FROM DROSOPHILA''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NDL is a [ | + | 1NDL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NDL OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the Awd nucleotide diphosphate kinase from Drosophila., Chiadmi M, Morera S, Lascu I, Dumas C, Le Bras G, Veron M, Janin J, Structure. 1993 Dec 15;1(4):283-93. PMID:[http:// | + | Crystal structure of the Awd nucleotide diphosphate kinase from Drosophila., Chiadmi M, Morera S, Lascu I, Dumas C, Le Bras G, Veron M, Janin J, Structure. 1993 Dec 15;1(4):283-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8081741 8081741] |
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Nucleoside-diphosphate kinase]] | [[Category: Nucleoside-diphosphate kinase]] | ||
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[[Category: phosphotransferase]] | [[Category: phosphotransferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:55:31 2008'' |
Revision as of 10:55, 20 March 2008
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| , resolution 2.4Å | |||||||
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| Activity: | Nucleoside-diphosphate kinase, with EC number 2.7.4.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE AWD NUCLEOTIDE DIPHOSPHATE KINASE FROM DROSOPHILA
Overview
BACKGROUND: Nucleotide diphosphate kinase (NDP kinase) is a phosphate transfer enzyme involved in cell regulation and in animal development. Drosophila NDP kinase is the product of the abnormal wing disc (awd) developmental gene, a point mutation in which can produce the killer of prune (K-pn) conditional lethal phenotype. The highly homologous mammalian genes control metastasis and a human NDP kinase acts as a transcription factor. RESULTS: The X-ray structure of the Awd protein prepared from Drosophila was solved at 2.4 A resolution by molecular replacement from the homologous Dictyostelium protein. Both are hexamers, and both have the same fold and the same active site. Subunit contacts differ as a result of sequence changes in the carboxy-terminal segment and in the loop that is the site of the K-pn mutation. CONCLUSIONS: Regulatory properties of animal NDP kinases depend on interactions with other macromolecules, such as DNA and the product of the Drosophila prune gene. The Awd structure suggests an allosteric mechanism of action of NDP kinase where DNA is the effector and the protein undergoes a major conformational change, possibly dissociating to dimers.
About this Structure
1NDL is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Awd nucleotide diphosphate kinase from Drosophila., Chiadmi M, Morera S, Lascu I, Dumas C, Le Bras G, Veron M, Janin J, Structure. 1993 Dec 15;1(4):283-93. PMID:8081741
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