3py3
From Proteopedia
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| - | [[ | + | ==Crystal structure of phosphorylated p38alpha MAP kinase== |
| + | <StructureSection load='3py3' size='340' side='right' caption='[[3py3]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3py3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PY3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PY3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mapk14, Crk1, Csbp1, Csbp2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mitogen-activated_protein_kinase Mitogen-activated protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.24 2.7.11.24] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3py3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3py3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3py3 RCSB], [http://www.ebi.ac.uk/pdbsum/3py3 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | MAPK phosphatase 3 (MKP3) is highly specific for ERK1/2 inactivation via dephosphorylation of both phosphotyrosine and phosphothreonine critical for enzymatic activation. Here, we show that MKP3 is able to effectively dephosphorylate the phosphotyrosine, but not phosphothreonine, in the activation loop of p38alpha in vitro and in intact cells. The catalytic constant of the MKP3 reaction for p38alpha is comparable with that for ERK2. Remarkably, MKP3, ERK2, and phosphorylated p38alpha can form a stable ternary complex in solution, and the phosphatase activity of MKP3 toward p38alpha substrate is allosterically regulated by ERK2-MKP3 interaction. This suggests that MKP3 not only controls the activities of ERK2 and p38alpha but also mediates cross-talk between these two MAPK pathways. The crystal structure of bisphosphorylated p38alpha has been determined at 2.1 A resolution. Comparisons between the phosphorylated MAPK structures reveal the molecular basis of MKP3 substrate specificity. | ||
| - | { | + | Mitogen-activated Protein Kinase (MAPK) Phosphatase 3-mediated Cross-talk between MAPKs ERK2 and p38{alpha}.,Zhang YY, Wu JW, Wang ZX J Biol Chem. 2011 May 6;286(18):16150-62. Epub 2011 Mar 16. PMID:21454500<ref>PMID:21454500</ref> |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Mitogen-activated protein kinase|Mitogen-activated protein kinase]] | *[[Mitogen-activated protein kinase|Mitogen-activated protein kinase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Mitogen-activated protein kinase]] | [[Category: Mitogen-activated protein kinase]] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
| - | [[Category: Wang, Z X | + | [[Category: Wang, Z X]] |
| - | [[Category: Wu, J W | + | [[Category: Wu, J W]] |
| - | [[Category: Zhang, Y Y | + | [[Category: Zhang, Y Y]] |
[[Category: Kinase]] | [[Category: Kinase]] | ||
[[Category: Phosphorylation]] | [[Category: Phosphorylation]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 12:28, 9 December 2014
Crystal structure of phosphorylated p38alpha MAP kinase
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