1ne7
From Proteopedia
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| - | [[Image:1ne7.gif|left|200px]] | + | [[Image:1ne7.gif|left|200px]] |
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| - | '''HUMAN GLUCOSAMINE-6-PHOSPHATE DEAMINASE ISOMERASE AT 1.75 A RESOLUTION COMPLEXED WITH N-ACETYL-GLUCOSAMINE-6-PHOSPHATE AND 2-DEOXY-2-AMINO-GLUCITOL-6-PHOSPHATE''' | + | {{Structure |
| + | |PDB= 1ne7 |SIZE=350|CAPTION= <scene name='initialview01'>1ne7</scene>, resolution 1.75Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=16G:N-ACETYL-D-GLUCOSAMINE-6-PHOSPHATE'>16G</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=AGP:2-DEOXY-2-AMINO GLUCITOL-6-PHOSPHATE'>AGP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Glucosamine-6-phosphate_deaminase Glucosamine-6-phosphate deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.99.6 3.5.99.6] | ||
| + | |GENE= GNPI OR HLN OR KIAA0060 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''HUMAN GLUCOSAMINE-6-PHOSPHATE DEAMINASE ISOMERASE AT 1.75 A RESOLUTION COMPLEXED WITH N-ACETYL-GLUCOSAMINE-6-PHOSPHATE AND 2-DEOXY-2-AMINO-GLUCITOL-6-PHOSPHATE''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NE7 is a [ | + | 1NE7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry 1D9T. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NE7 OCA]. |
==Reference== | ==Reference== | ||
| - | Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study., Arreola R, Valderrama B, Morante ML, Horjales E, FEBS Lett. 2003 Sep 11;551(1-3):63-70. PMID:[http:// | + | Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study., Arreola R, Valderrama B, Morante ML, Horjales E, FEBS Lett. 2003 Sep 11;551(1-3):63-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12965206 12965206] |
[[Category: Glucosamine-6-phosphate deaminase]] | [[Category: Glucosamine-6-phosphate deaminase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: GLC]] | [[Category: GLC]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
| - | [[Category: conformational | + | [[Category: conformational difference]] |
[[Category: conformational disorder]] | [[Category: conformational disorder]] | ||
[[Category: v-type like allosteric enzyme]] | [[Category: v-type like allosteric enzyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:55:42 2008'' |
Revision as of 10:55, 20 March 2008
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| , resolution 1.75Å | |||||||
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| Ligands: | , , and | ||||||
| Gene: | GNPI OR HLN OR KIAA0060 (Homo sapiens) | ||||||
| Activity: | Glucosamine-6-phosphate deaminase, with EC number 3.5.99.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HUMAN GLUCOSAMINE-6-PHOSPHATE DEAMINASE ISOMERASE AT 1.75 A RESOLUTION COMPLEXED WITH N-ACETYL-GLUCOSAMINE-6-PHOSPHATE AND 2-DEOXY-2-AMINO-GLUCITOL-6-PHOSPHATE
Contents |
Overview
Glucosamine-6-phosphate deaminase (EC 3.5.99.6) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. Here we describe the existence of two mammalian glucosamine-6-phosphate deaminase enzymes. We present the crystallographic structure of one of them, the long human glucosamine-6-phosphate deaminase, at 1.75 A resolution. Crystals belong to the space group P2(1)2(1)2(1) and present a whole hexamer in the asymmetric unit. The active-site lid (residues 162-182) presented significant structural differences among monomers. Interestingly the region with the largest differences, when compared with the Escherichia coli homologue, was found to be close to the active site. These structural differences can be related to the kinetic and allosteric properties of both mammalian enzymes.
Disease
Known disease associated with this structure: Huntington disease-like 3 OMIM:[604802]
About this Structure
1NE7 is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 1D9T. Full crystallographic information is available from OCA.
Reference
Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study., Arreola R, Valderrama B, Morante ML, Horjales E, FEBS Lett. 2003 Sep 11;551(1-3):63-70. PMID:12965206
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