1ne9
From Proteopedia
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| - | [[Image:1ne9.jpg|left|200px]] | + | [[Image:1ne9.jpg|left|200px]] |
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| - | '''Crystal Structure of Weissella viridescens FemX at 1.7 Ang Resolution''' | + | {{Structure |
| + | |PDB= 1ne9 |SIZE=350|CAPTION= <scene name='initialview01'>1ne9</scene>, resolution 1.70Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= femx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1629 Weissella viridescens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Weissella viridescens FemX at 1.7 Ang Resolution''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NE9 is a [ | + | 1NE9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Weissella_viridescens Weissella viridescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NE9 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures of Weissella viridescens FemX and its complex with UDP-MurNAc-pentapeptide: insights into FemABX family substrates recognition., Biarrotte-Sorin S, Maillard AP, Delettre J, Sougakoff W, Arthur M, Mayer C, Structure. 2004 Feb;12(2):257-67. PMID:[http:// | + | Crystal structures of Weissella viridescens FemX and its complex with UDP-MurNAc-pentapeptide: insights into FemABX family substrates recognition., Biarrotte-Sorin S, Maillard AP, Delettre J, Sougakoff W, Arthur M, Mayer C, Structure. 2004 Feb;12(2):257-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14962386 14962386] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Weissella viridescens]] | [[Category: Weissella viridescens]] | ||
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[[Category: protein]] | [[Category: protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:55:44 2008'' |
Revision as of 10:55, 20 March 2008
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| , resolution 1.70Å | |||||||
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| Ligands: | |||||||
| Gene: | femx (Weissella viridescens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Weissella viridescens FemX at 1.7 Ang Resolution
Overview
Members of the FemABX protein family are novel therapeutic targets, as they are involved in the synthesis of the bacterial cell wall. They catalyze the addition of amino acid(s) on the peptidoglycan precursor using aminoacylated tRNA as a substrate. We report here the high-resolution structure of Weissella viridescens L-alanine transferase FemX and its complex with the UDP-MurNAc-pentapeptide. This is the first structure example of a FemABX family member that does not possess a coiled-coil domain. FemX consists of two structurally equivalent domains, separated by a cleft containing the binding site of the UDP-MurNAc-pentapeptide and a long channel that traverses one of the two domains. Our structural studies bring new insights into the evolution of the FemABX and the related GNAT superfamilies, shed light on the recognition site of the aminoacylated tRNA in Fem proteins, and allowed manual docking of the acceptor end of the alanyl-tRNAAla.
About this Structure
1NE9 is a Single protein structure of sequence from Weissella viridescens. Full crystallographic information is available from OCA.
Reference
Crystal structures of Weissella viridescens FemX and its complex with UDP-MurNAc-pentapeptide: insights into FemABX family substrates recognition., Biarrotte-Sorin S, Maillard AP, Delettre J, Sougakoff W, Arthur M, Mayer C, Structure. 2004 Feb;12(2):257-67. PMID:14962386
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