This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1xmo

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1xmo.png|left|200px]]
+
==Crystal Structure of mnm5U34t6A37-tRNALysUUU Complexed with AAG-mRNA in the Decoding Center==
 +
<StructureSection load='1xmo' size='340' side='right' caption='[[1xmo]], [[Resolution|resolution]] 3.25&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1xmo]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XMO FirstGlance]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
 +
<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MNU:(2R,4S)-1-[(4R)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]-5-[(METHYLAMINO)METHYL]-1,2,3,4-TETRAHYDROPYRIMIDINE-2,4-DIOL-5-MONOPHOSPHATE'>MNU</scene>, <scene name='pdbligand=T6A:N-[N-(9-B-D-RIBOFURANOSYLPURIN-6-YL)CARBAMOYL]THREONINE-5-MONOPHOSPHATE'>T6A</scene></td></tr>
 +
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ibl|1ibl]], [[1ibk|1ibk]], [[1ibm|1ibm]], [[1fka|1fka]], [[1xmq|1xmq]], [[1xnq|1xnq]], [[1xnr|1xnr]]</td></tr>
 +
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xmo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1xmo RCSB], [http://www.ebi.ac.uk/pdbsum/1xmo PDBsum]</span></td></tr>
 +
<table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xm/1xmo_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The natural modification of specific nucleosides in many tRNAs is essential during decoding of mRNA by the ribosome. For example, tRNA(Lys)(UUU) requires the modification N6-threonylcarbamoyladenosine at position 37 (t(6)A37), adjacent and 3' to the anticodon, to bind AAA in the A site of the ribosomal 30S subunit. Moreover, it can only bind both AAA and AAG lysine codons when doubly modified with t(6)A37 and either 5-methylaminomethyluridine or 2-thiouridine at the wobble position (mnm(5)U34 or s(2)U34). Here we report crystal structures of modified tRNA anticodon stem-loops bound to the 30S ribosomal subunit with lysine codons in the A site. These structures allow the rationalization of how modifications in the anticodon loop enable decoding of both lysine codons AAA and AAG.
-
{{STRUCTURE_1xmo| PDB=1xmo | SCENE= }}
+
The role of modifications in codon discrimination by tRNA(Lys)UUU.,Murphy FV 4th, Ramakrishnan V, Malkiewicz A, Agris PF Nat Struct Mol Biol. 2004 Dec;11(12):1186-91. Epub 2004 Nov 21. PMID:15558052<ref>PMID:15558052</ref>
-
===Crystal Structure of mnm5U34t6A37-tRNALysUUU Complexed with AAG-mRNA in the Decoding Center===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_15558052}}
+
-
 
+
-
==About this Structure==
+
-
[[1xmo]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XMO OCA].
+
==See Also==
==See Also==
-
*[[Ribosomal protein S10|Ribosomal protein S10]]
 
-
*[[Ribosomal protein S11|Ribosomal protein S11]]
 
-
*[[Ribosomal protein S12|Ribosomal protein S12]]
 
-
*[[Ribosomal protein S13|Ribosomal protein S13]]
 
-
*[[Ribosomal protein S14|Ribosomal protein S14]]
 
-
*[[Ribosomal protein S15|Ribosomal protein S15]]
 
-
*[[Ribosomal protein S16|Ribosomal protein S16]]
 
-
*[[Ribosomal protein S17|Ribosomal protein S17]]
 
-
*[[Ribosomal protein S18|Ribosomal protein S18]]
 
-
*[[Ribosomal protein S19|Ribosomal protein S19]]
 
-
*[[Ribosomal protein S2|Ribosomal protein S2]]
 
-
*[[Ribosomal protein S20|Ribosomal protein S20]]
 
-
*[[Ribosomal protein S3|Ribosomal protein S3]]
 
-
*[[Ribosomal protein S4|Ribosomal protein S4]]
 
-
*[[Ribosomal protein S5|Ribosomal protein S5]]
 
-
*[[Ribosomal protein S6|Ribosomal protein S6]]
 
-
*[[Ribosomal protein S7|Ribosomal protein S7]]
 
-
*[[Ribosomal protein S8|Ribosomal protein S8]]
 
-
*[[Ribosomal protein S9|Ribosomal protein S9]]
 
*[[Ribosomal protein THX|Ribosomal protein THX]]
*[[Ribosomal protein THX|Ribosomal protein THX]]
-
 
+
*[[Ribosome 3D structures|Ribosome 3D structures]]
-
==Reference==
+
== References ==
-
<ref group="xtra">PMID:015558052</ref><references group="xtra"/>
+
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
[[Category: Agris, P F.]]
[[Category: Agris, P F.]]

Revision as of 00:07, 29 September 2014

Crystal Structure of mnm5U34t6A37-tRNALysUUU Complexed with AAG-mRNA in the Decoding Center

1xmo, resolution 3.25Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xmo"
Views
Personal tools
Navigation
Toolbox