1ng4

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[[Image:1ng4.jpg|left|200px]]<br /><applet load="1ng4" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ng4.jpg|left|200px]]
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caption="1ng4, resolution 2.30&Aring;" />
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'''Structure of ThiO (glycine oxidase) from Bacillus subtilis'''<br />
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{{Structure
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|PDB= 1ng4 |SIZE=350|CAPTION= <scene name='initialview01'>1ng4</scene>, resolution 2.30&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=PEO:HYDROGEN PEROXIDE'>PEO</scene>
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|ACTIVITY=
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|GENE= GOXB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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}}
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'''Structure of ThiO (glycine oxidase) from Bacillus subtilis'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1NG4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=PEO:'>PEO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NG4 OCA].
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1NG4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NG4 OCA].
==Reference==
==Reference==
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Structural and mechanistic studies on ThiO, a glycine oxidase essential for thiamin biosynthesis in Bacillus subtilis., Settembre EC, Dorrestein PC, Park JH, Augustine AM, Begley TP, Ealick SE, Biochemistry. 2003 Mar 18;42(10):2971-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12627963 12627963]
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Structural and mechanistic studies on ThiO, a glycine oxidase essential for thiamin biosynthesis in Bacillus subtilis., Settembre EC, Dorrestein PC, Park JH, Augustine AM, Begley TP, Ealick SE, Biochemistry. 2003 Mar 18;42(10):2971-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12627963 12627963]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: oxidase]]
[[Category: oxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:56:26 2008''

Revision as of 10:56, 20 March 2008

Image:1ng4.jpg


PDB ID 1ng4

Drag the structure with the mouse to rotate
, resolution 2.30Å
Ligands: , and
Gene: GOXB (Bacillus subtilis)
Coordinates: save as pdb, mmCIF, xml



Structure of ThiO (glycine oxidase) from Bacillus subtilis


Overview

The thiO gene of Bacillus subtilis encodes an FAD-dependent glycine oxidase. This enzyme is a homotetramer with a monomer molecular mass of 42 kDa. In this paper, we demonstrate that ThiO is required for the biosynthesis of the thiazole moiety of thiamin pyrophosphate and describe the structure of the enzyme with N-acetylglycine bound at the active site. The closest structural relatives of ThiO are sarcosine oxidase and d-amino acid oxidase. The ThiO structure, as well as the observation that N-cyclopropylglycine is a good substrate, supports a hydride transfer mechanism for the enzyme. A mechanistic proposal for the role of ThiO in thiazole biosynthesis is also described.

About this Structure

1NG4 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Structural and mechanistic studies on ThiO, a glycine oxidase essential for thiamin biosynthesis in Bacillus subtilis., Settembre EC, Dorrestein PC, Park JH, Augustine AM, Begley TP, Ealick SE, Biochemistry. 2003 Mar 18;42(10):2971-81. PMID:12627963

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