1nhc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1nhc.gif|left|200px]]<br /><applet load="1nhc" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1nhc.gif|left|200px]]
-
caption="1nhc, resolution 1.70&Aring;" />
+
 
-
'''Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger'''<br />
+
{{Structure
 +
|PDB= 1nhc |SIZE=350|CAPTION= <scene name='initialview01'>1nhc</scene>, resolution 1.70&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Polygalacturonase Polygalacturonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.15 3.2.1.15]
 +
|GENE=
 +
}}
 +
 
 +
'''Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1NHC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger] with <scene name='pdbligand=MAN:'>MAN</scene>, <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Polygalacturonase Polygalacturonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.15 3.2.1.15] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHC OCA].
+
1NHC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHC OCA].
==Reference==
==Reference==
-
Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger., van Pouderoyen G, Snijder HJ, Benen JA, Dijkstra BW, FEBS Lett. 2003 Nov 20;554(3):462-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14623112 14623112]
+
Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger., van Pouderoyen G, Snijder HJ, Benen JA, Dijkstra BW, FEBS Lett. 2003 Nov 20;554(3):462-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14623112 14623112]
[[Category: Aspergillus niger]]
[[Category: Aspergillus niger]]
[[Category: Polygalacturonase]]
[[Category: Polygalacturonase]]
Line 24: Line 33:
[[Category: beta-helix]]
[[Category: beta-helix]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:10 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:56:54 2008''

Revision as of 10:56, 20 March 2008

Image:1nhc.gif


PDB ID 1nhc

Drag the structure with the mouse to rotate
, resolution 1.70Å
Ligands: , , and
Activity: Polygalacturonase, with EC number 3.2.1.15
Coordinates: save as pdb, mmCIF, xml



Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger


Overview

Endopolygalacturonase I is a processive enzyme, while the 60% sequence identical endopolygalacturonase II is not. The 1.70 A resolution crystal structure of endopolygalacturonase I reveals a narrowed substrate binding cleft. In addition, Arg96, a residue in this cleft previously shown to be critical for processivity, interacts with the substrate mimics glycerol and sulfate in several well-defined conformations in the six molecules in the asymmetric unit. From this we conclude that both Arg96 and the narrowed substrate binding cleft contribute to retaining the substrate while it moves through the active site after a cleavage event has occurred.

About this Structure

1NHC is a Single protein structure of sequence from Aspergillus niger. Full crystallographic information is available from OCA.

Reference

Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger., van Pouderoyen G, Snijder HJ, Benen JA, Dijkstra BW, FEBS Lett. 2003 Nov 20;554(3):462-6. PMID:14623112

Page seeded by OCA on Thu Mar 20 12:56:54 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nhc"
Personal tools