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1nio

From Proteopedia

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[[Image:1nio.gif|left|200px]]<br /><applet load="1nio" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1nio.gif|left|200px]]
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caption="1nio, resolution 2.00&Aring;" />
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'''Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution'''<br />
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{{Structure
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|PDB= 1nio |SIZE=350|CAPTION= <scene name='initialview01'>1nio</scene>, resolution 2.00&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22]
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|GENE=
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}}
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'''Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1NIO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Luffa_aegyptiaca Luffa aegyptiaca] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NIO OCA].
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1NIO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Luffa_aegyptiaca Luffa aegyptiaca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NIO OCA].
==Reference==
==Reference==
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Crystal structure of beta-luffin, a ribosome-inactivating protein, at 2.0 A resolution., Ma QJ, Li JH, Li HG, Wu S, Dong YC, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1366-70. Epub 2003, Jul 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12876337 12876337]
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Crystal structure of beta-luffin, a ribosome-inactivating protein, at 2.0 A resolution., Ma QJ, Li JH, Li HG, Wu S, Dong YC, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1366-70. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876337 12876337]
[[Category: Luffa aegyptiaca]]
[[Category: Luffa aegyptiaca]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: ribosome inactivating protein(rip)]]
[[Category: ribosome inactivating protein(rip)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:57:28 2008''

Revision as of 10:57, 20 March 2008


PDB ID 1nio

Drag the structure with the mouse to rotate
, resolution 2.00Å
Ligands:
Activity: rRNA N-glycosylase, with EC number 3.2.2.22
Coordinates: save as pdb, mmCIF, xml



Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution


Overview

The crystal structure of beta-luffin at 2.0 A resolution was solved by the molecular-replacement method using polyalanyl trichosanthin as the search model. The structure was refined with CNS1.1, giving R(work) = 0.162 and R(free) = 0.204. The r.m.s.d.s of the bond lengths and bond angles are 0.008 A and 1.3 degrees, respectively. The overall structure is similar to those of other type I RIPs. Three N-acetylglucosamine (Nag) molecules are linked to residues Asn2, Asn78 and Asn85 of the protein.

About this Structure

1NIO is a Single protein structure of sequence from Luffa aegyptiaca. Full crystallographic information is available from OCA.

Reference

Crystal structure of beta-luffin, a ribosome-inactivating protein, at 2.0 A resolution., Ma QJ, Li JH, Li HG, Wu S, Dong YC, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1366-70. Epub 2003, Jul 23. PMID:12876337

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