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2xqd
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| - | [[ | + | ==THE STRUCTURE OF EF-TU AND AMINOACYL-TRNA BOUND TO THE 70S RIBOSOME WITH A GTP ANALOG== |
| + | <StructureSection load='2xqd' size='340' side='right' caption='[[2xqd]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2xqd]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XQD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XQD FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xqe|2xqe]], [[1dk1|1dk1]], [[1ibk|1ibk]], [[1f7y|1f7y]], [[1lou|1lou]], [[1ibl|1ibl]], [[2uuc|2uuc]], [[2wdk|2wdk]], [[1kuq|1kuq]], [[2c77|2c77]], [[1ab3|1ab3]], [[2j02|2j02]], [[1qjh|1qjh]], [[1j5e|1j5e]], [[2wrq|2wrq]], [[2b9o|2b9o]], [[2j00|2j00]], [[1hnx|1hnx]], [[2x9t|2x9t]], [[2wh3|2wh3]], [[2uub|2uub]], [[1mvr|1mvr]], [[1cqn|1cqn]], [[1emw|1emw]], [[2v46|2v46]], [[1pnx|1pnx]], [[1qkf|1qkf]], [[1qkh|1qkh]], [[2vqf|2vqf]], [[2wh1|2wh1]], [[1n32|1n32]], [[2wrn|2wrn]], [[2jl5|2jl5]], [[2f4v|2f4v]], [[1twt|1twt]], [[2uxd|2uxd]], [[1fka|1fka]], [[1n36|1n36]], [[2uxc|2uxc]], [[1fjg|1fjg]], [[1hnw|1hnw]], [[1l1u|1l1u]], [[1hr0|1hr0]], [[2v48|2v48]], [[1an7|1an7]], [[2b64|2b64]], [[1xmq|1xmq]], [[2b9m|2b9m]], [[2wdg|2wdg]], [[1xnr|1xnr]], [[1hnz|1hnz]], [[2wdh|2wdh]], [[1jgq|1jgq]], [[1i94|1i94]], [[1cqm|1cqm]], [[1i96|1i96]], [[2uu9|2uu9]], [[1eg0|1eg0]], [[1vox|1vox]], [[1ibm|1ibm]], [[2uxb|2uxb]], [[2vqe|2vqe]], [[2bvz|2bvz]], [[1i95|1i95]], [[2uua|2uua]], [[1voz|1voz]], [[1xnq|1xnq]], [[2xfz|2xfz]], [[2bxj|2bxj]], [[1qd7|1qd7]], [[1gix|1gix]], [[1jgo|1jgo]], [[1jgp|1jgp]], [[2xg1|2xg1]], [[2x9r|2x9r]], [[1n34|1n34]], [[1n33|1n33]], [[1pns|1pns]], [[2jl7|2jl7]], [[1yl4|1yl4]], [[1ris|1ris]], [[2wdm|2wdm]], [[1xmo|1xmo]], [[1i97|1i97]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xqd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xqd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xqd RCSB], [http://www.ebi.ac.uk/pdbsum/2xqd PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Protein synthesis requires several guanosine triphosphatase (GTPase) factors, including elongation factor Tu (EF-Tu), which delivers aminoacyl-transfer RNAs (tRNAs) to the ribosome. To understand how the ribosome triggers GTP hydrolysis in translational GTPases, we have determined the crystal structure of EF-Tu and aminoacyl-tRNA bound to the ribosome with a GTP analog, to 3.2 angstrom resolution. EF-Tu is in its active conformation, the switch I loop is ordered, and the catalytic histidine is coordinating the nucleophilic water in position for inline attack on the gamma-phosphate of GTP. This activated conformation is due to a critical and conserved interaction of the histidine with A2662 of the sarcin-ricin loop of the 23S ribosomal RNA. The structure suggests a universal mechanism for GTPase activation and hydrolysis in translational GTPases on the ribosome. | ||
| - | + | The mechanism for activation of GTP hydrolysis on the ribosome.,Voorhees RM, Schmeing TM, Kelley AC, Ramakrishnan V Science. 2010 Nov 5;330(6005):835-8. PMID:21051640<ref>PMID:21051640</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Elongation factor|Elongation factor]] | *[[Elongation factor|Elongation factor]] | ||
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
Revision as of 13:07, 22 October 2014
THE STRUCTURE OF EF-TU AND AMINOACYL-TRNA BOUND TO THE 70S RIBOSOME WITH A GTP ANALOG
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