1nlt
From Proteopedia
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| - | [[Image:1nlt.jpg|left|200px]] | + | [[Image:1nlt.jpg|left|200px]] |
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| - | '''The crystal structure of Hsp40 Ydj1''' | + | {{Structure |
| + | |PDB= 1nlt |SIZE=350|CAPTION= <scene name='initialview01'>1nlt</scene>, resolution 2.70Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= MAS5 OR YDJ1 OR YNL064C OR N2418 OR YNL2418C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''The crystal structure of Hsp40 Ydj1''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NLT is a [ | + | 1NLT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NLT OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of the yeast Hsp40 Ydj1 complexed with its peptide substrate., Li J, Qian X, Sha B, Structure. 2003 Dec;11(12):1475-83. PMID:[http:// | + | The crystal structure of the yeast Hsp40 Ydj1 complexed with its peptide substrate., Li J, Qian X, Sha B, Structure. 2003 Dec;11(12):1475-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14656432 14656432] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Sha, B.]] | [[Category: Sha, B.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| - | [[Category: beta- | + | [[Category: beta-strand]] |
[[Category: chaperone]] | [[Category: chaperone]] | ||
[[Category: heat shock]] | [[Category: heat shock]] | ||
[[Category: mitochondrion]] | [[Category: mitochondrion]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:58:32 2008'' |
Revision as of 10:58, 20 March 2008
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| , resolution 2.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | MAS5 OR YDJ1 OR YNL064C OR N2418 OR YNL2418C (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The crystal structure of Hsp40 Ydj1
Overview
The mechanisms by which Hsp40 functions as a molecular chaperone to recognize and bind non-native polypeptides is not understood. We have identified a peptide substrate for Ydj1, a member of the type I Hsp40 from yeast. The structure of the Ydj1 peptide binding fragment and its peptide substrate complex was determined to 2.7 A resolution. The complex structure reveals that Ydj1 peptide binding fragment forms an L-shaped molecule constituted by three domains. The domain I exhibits a similar protein folds as domain III while the domain II contains two Zinc finger motifs. The peptide substrate binds Ydj1 by forming an extra beta strand with domain I of Ydj1. The Leucine residue in the middle of the peptide substrate GWLYEIS inserts its side chain into a hydrophobic pocket formed on the molecular surface of Ydj1 domain I. The Zinc finger motifs located in the Ydj1 domain II are not in the vicinity of peptide substrate binding site.
About this Structure
1NLT is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The crystal structure of the yeast Hsp40 Ydj1 complexed with its peptide substrate., Li J, Qian X, Sha B, Structure. 2003 Dec;11(12):1475-83. PMID:14656432
Page seeded by OCA on Thu Mar 20 12:58:32 2008
