3sfg

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[[Image:3sfg.png|left|200px]]
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==crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)==
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<StructureSection load='3sfg' size='340' side='right' caption='[[3sfg]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3sfg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SFG FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2TU:1-(BETA-D-RIBOFURANOSYL)-2-THIOXO-2,3-DIHYDROPYRIMIDIN-4(1H)-ONE'>2TU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nah|3nah]], [[3nai|3nai]], [[3qid|3qid]], [[3sfu|3sfu]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RdRp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223997 Murine norovirus 1])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sfg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sfg RCSB], [http://www.ebi.ac.uk/pdbsum/3sfg PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Murine norovirus-1 (MNV-1) shares many features with human norovirus (HuNoV) and both are classified within the norovirus genus of Caliciviridae family. MNV-1 is used as the surrogate for HuNoV research since it is the only form that can be grown in cell culture. HuNoV and MNV-1 RNA dependent RNA polymerase (RdRp) proteins with the sequence identity of 59% show essentially identical conformations. Here we report the first structural evidence of 2-thiouridine (2TU) or ribavirin binding to MNV-1 RdRp, based on the crystal structures determined at 2.2A and 2.5A resolutions, respectively. Cellular and biochemical studies revealed stronger inhibitory effect of 2TU on the replication of MNV-1 in RAW 264.7 cells, compared to that of ribavirin. Our complex structures highlight the key interactions involved in recognition of the nucleoside analogs which block the active site of the viral RNA polymerase.
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{{STRUCTURE_3sfg| PDB=3sfg | SCENE= }}
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Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase in complex with 2-thiouridine or ribavirin.,Alam I, Lee JH, Cho KJ, Han KR, Yang JM, Chung MS, Kim KH Virology. 2012 May 10;426(2):143-51. Epub 2012 Feb 16. PMID:22341781<ref>PMID:22341781</ref>
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===crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_22341781}}
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==About this Structure==
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[[3sfg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFG OCA].
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==See Also==
==See Also==
*[[RNA polymerase|RNA polymerase]]
*[[RNA polymerase|RNA polymerase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:022341781</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Murine norovirus 1]]
[[Category: Murine norovirus 1]]
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[[Category: Alam, I.]]
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[[Category: Alam, I]]
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[[Category: Kim, K H.]]
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[[Category: Kim, K H]]
[[Category: Polymerase]]
[[Category: Polymerase]]
[[Category: Right hand conformation]]
[[Category: Right hand conformation]]
[[Category: Rna binding]]
[[Category: Rna binding]]
[[Category: Transferase]]
[[Category: Transferase]]

Revision as of 10:54, 9 December 2014

crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)

3sfg, resolution 2.21Å

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