2a3p
From Proteopedia
(Difference between revisions)
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| - | [[ | + | ==Structure of Desulfovibrio desulfuricans G20 tetraheme cytochrome with bound molybdate== |
| + | <StructureSection load='2a3p' size='340' side='right' caption='[[2a3p]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2a3p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_alaskensis_g20 Desulfovibrio alaskensis g20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A3P FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a3p RCSB], [http://www.ebi.ac.uk/pdbsum/2a3p PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/2a3p_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The structure of the type I tetraheme cytochrome c(3) from Desulfovibrio desulfuricans G20 was determined to 1.5 Angstrom by X-ray crystallography. In addition to the oxidized form, the structure of the molybdate-bound form of the protein was determined from oxidized crystals soaked in sodium molybdate. Only small structural shifts were obtained with metal binding, consistent with the remarkable structural stability of this protein. In vitro experiments with pure cytochrome showed that molybdate could oxidize the reduced cytochrome, although not as rapidly as U(VI) present as uranyl acetate. Alterations in the overall conformation and thermostability of the metal-oxidized protein were investigated by circular dichroism studies. Again, only small changes in protein structure were documented. The location of the molybdate ion near heme IV in the crystal structure suggested heme IV as the site of electron exit from the reduced cytochrome and implicated Lys14 and Lys56 in binding. Analysis of structurally conserved water molecules in type I cytochrome c(3) crystal structures identified interactions predicted to be important for protein stability and possibly for intramolecular electron transfer among heme molecules. | ||
| - | + | Desulfovibrio desulfuricans G20 tetraheme cytochrome structure at 1.5 Angstrom and cytochrome interaction with metal complexes.,Pattarkine MV, Tanner JJ, Bottoms CA, Lee YH, Wall JD J Mol Biol. 2006 May 19;358(5):1314-27. Epub 2006 Mar 23. PMID:16580681<ref>PMID:16580681</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Cytochrome c|Cytochrome c]] | *[[Cytochrome c|Cytochrome c]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Desulfovibrio alaskensis g20]] | [[Category: Desulfovibrio alaskensis g20]] | ||
| - | [[Category: Lee, Y H | + | [[Category: Lee, Y H]] |
| - | [[Category: Pattarkine, M V | + | [[Category: Pattarkine, M V]] |
| - | [[Category: Tanner, J J | + | [[Category: Tanner, J J]] |
| - | [[Category: Wall, J D | + | [[Category: Wall, J D]] |
[[Category: Cytochrome c3]] | [[Category: Cytochrome c3]] | ||
[[Category: Desulfovibrio]] | [[Category: Desulfovibrio]] | ||
[[Category: Electron transport]] | [[Category: Electron transport]] | ||
[[Category: Tetraheme cytochrome]] | [[Category: Tetraheme cytochrome]] | ||
Revision as of 09:55, 8 January 2015
Structure of Desulfovibrio desulfuricans G20 tetraheme cytochrome with bound molybdate
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