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1nth
From Proteopedia
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| - | [[Image:1nth.gif|left|200px]] | + | [[Image:1nth.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure of the methanosarcina barkeri monomethylamine methyltransferase (MTMB)''' | + | {{Structure |
| + | |PDB= 1nth |SIZE=350|CAPTION= <scene name='initialview01'>1nth</scene>, resolution 1.55Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=BGX:4-METHYL-PYRROLINE-5-CARBOXYLIC ACID'>BGX</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the methanosarcina barkeri monomethylamine methyltransferase (MTMB)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NTH is a [ | + | 1NTH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_barkeri Methanosarcina barkeri]. This structure supersedes the now removed PDB entry 1L2R. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTH OCA]. |
==Reference== | ==Reference== | ||
| - | A new UAG-encoded residue in the structure of a methanogen methyltransferase., Hao B, Gong W, Ferguson TK, James CM, Krzycki JA, Chan MK, Science. 2002 May 24;296(5572):1462-6. PMID:[http:// | + | A new UAG-encoded residue in the structure of a methanogen methyltransferase., Hao B, Gong W, Ferguson TK, James CM, Krzycki JA, Chan MK, Science. 2002 May 24;296(5572):1462-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12029132 12029132] |
[[Category: Methanosarcina barkeri]] | [[Category: Methanosarcina barkeri]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: tim barrel]] | [[Category: tim barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:01:28 2008'' |
Revision as of 11:01, 20 March 2008
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| , resolution 1.55Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the methanosarcina barkeri monomethylamine methyltransferase (MTMB)
Overview
Genes encoding methanogenic methylamine methyltransferases all contain an in-frame amber (UAG) codon that is read through during translation. We have identified the UAG-encoded residue in a 1.55 angstrom resolution structure of the Methanosarcina barkeri monomethylamine methyltransferase (MtmB). This structure reveals a homohexamer comprised of individual subunits with a TIM barrel fold. The electron density for the UAG-encoded residue is distinct from any of the 21 natural amino acids. Instead it appears consistent with a lysine in amide-linkage to (4R,5R)-4-substituted-pyrroline-5-carboxylate. We suggest that this amino acid be named l-pyrrolysine.
About this Structure
1NTH is a Single protein structure of sequence from Methanosarcina barkeri. This structure supersedes the now removed PDB entry 1L2R. Full crystallographic information is available from OCA.
Reference
A new UAG-encoded residue in the structure of a methanogen methyltransferase., Hao B, Gong W, Ferguson TK, James CM, Krzycki JA, Chan MK, Science. 2002 May 24;296(5572):1462-6. PMID:12029132
Page seeded by OCA on Thu Mar 20 13:01:28 2008
