1nu4
From Proteopedia
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| - | [[Image:1nu4.gif|left|200px]] | + | [[Image:1nu4.gif|left|200px]] |
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| - | '''U1A RNA binding domain at 1.8 angstrom resolution reveals a pre-organized C-terminal helix''' | + | {{Structure |
| + | |PDB= 1nu4 |SIZE=350|CAPTION= <scene name='initialview01'>1nu4</scene>, resolution 1.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=MLA:MALONIC ACID'>MLA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''U1A RNA binding domain at 1.8 angstrom resolution reveals a pre-organized C-terminal helix''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NU4 is a [ | + | 1NU4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NU4 OCA]. |
==Reference== | ==Reference== | ||
| - | U1A RNA-binding domain at 1.8 A resolution., Rupert PB, Xiao H, Ferre-D'Amare AR, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1521-4. Epub 2003, Jul 23. PMID:[http:// | + | U1A RNA-binding domain at 1.8 A resolution., Rupert PB, Xiao H, Ferre-D'Amare AR, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1521-4. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876372 12876372] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: u1 small nuclear ribonucleoprotein]] | [[Category: u1 small nuclear ribonucleoprotein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:01:41 2008'' |
Revision as of 11:01, 20 March 2008
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| , resolution 1.80Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
U1A RNA binding domain at 1.8 angstrom resolution reveals a pre-organized C-terminal helix
Overview
The human U1A RNA-binding domain (RBD1) adopts one of the most common protein folds, the RNA-recognition motif, and is a paradigm for understanding RNA-protein interactions. A 2.8 A resolution structure of the unbound RBD1 has previously been determined [Nagai et al. (1990). Nature (London), 348, 515-520] and revealed a well defined alpha/beta core with disordered termini. Using a longer construct, a 1.8 A resolution structure of the unbound domain was determined that reveals an ordered C-terminal helix. The presence of this helix is consistent with a solution structure of the free domain [Avis et al. (1996). J. Mol. Biol. 257, 398-411]; however, in the solution structure the helix occludes the RNA-binding surface. In the present structure, the helix occupies a position similar to that seen in a 1.9 A resolution RNA-RBD1 complex structure [Oubridge et al. (1994). Nature (London), 372, 432-438]. The crystals in this study were grown from 2.2 M sodium malonate. It is possible that the high salt concentration helps to orient the C-terminal helix in the RNA-bound conformation by strengthening hydrophobic interactions between the buried face of the helix and the alpha/beta core of the protein. Alternatively, the malonate (several molecules of which are bound in the vicinity of the RNA-binding surface) may mimic RNA.
About this Structure
1NU4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
U1A RNA-binding domain at 1.8 A resolution., Rupert PB, Xiao H, Ferre-D'Amare AR, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1521-4. Epub 2003, Jul 23. PMID:12876372
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