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3v6d

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[[Image:3v6d.png|left|200px]]
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==Crystal structure of HIV-1 reverse transcriptase (RT) cross-linked with AZT-terminated DNA==
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<StructureSection load='3v6d' size='340' side='right' caption='[[3v6d]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3v6d]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_type_1_bh10 Human immunodeficiency virus type 1 bh10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V6D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3V6D FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATM:3-AZIDO-3-DEOXYTHYMIDINE-5-MONOPHOSPHATE'>ATM</scene>, <scene name='pdbligand=MRG:N2-(3-MERCAPTOPROPYL)-2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>MRG</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3v4i|3v4i]], [[3v81|3v81]], [[1rtd|1rtd]], [[2zd1|2zd1]], [[1vrt|1vrt]], [[3kle|3kle]], [[3klf|3klf]], [[3jsm|3jsm]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gag-pol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11678 Human immunodeficiency virus type 1 BH10])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3v6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v6d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3v6d RCSB], [http://www.ebi.ac.uk/pdbsum/3v6d PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Combinations of nucleoside and non-nucleoside inhibitors (NNRTIs) of HIV-1 reverse transcriptase (RT) are widely used in anti-AIDS therapies. Five NNRTIs, including nevirapine, are clinical drugs; however, the molecular mechanism of inhibition by NNRTIs is not clear. We determined the crystal structures of RT-DNA-nevirapine, RT-DNA, and RT-DNA-AZT-triphosphate complexes at 2.85-, 2.70- and 2.80-A resolution, respectively. The RT-DNA complex in the crystal could bind nevirapine or AZT-triphosphate but not both. Binding of nevirapine led to opening of the NNRTI-binding pocket. The pocket formation caused shifting of the 3' end of the DNA primer by ~5.5 A away from its polymerase active site position. Nucleic acid interactions with fingers and palm subdomains were reduced, the dNTP-binding pocket was distorted and the thumb opened up. The structures elucidate complementary roles of nucleoside and non-nucleoside inhibitors in inhibiting RT.
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{{STRUCTURE_3v6d| PDB=3v6d | SCENE= }}
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HIV-1 reverse transcriptase complex with DNA and nevirapine reveals non-nucleoside inhibition mechanism.,Das K, Martinez SE, Bauman JD, Arnold E Nat Struct Mol Biol. 2012 Jan 22;19(2):253-9. doi: 10.1038/nsmb.2223. PMID:22266819<ref>PMID:22266819</ref>
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===Crystal structure of HIV-1 reverse transcriptase (RT) cross-linked with AZT-terminated DNA===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_22266819}}
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==About this Structure==
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[[3v6d]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_type_1_bh10 Human immunodeficiency virus type 1 bh10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V6D OCA].
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==See Also==
==See Also==
*[[Reverse transcriptase|Reverse transcriptase]]
*[[Reverse transcriptase|Reverse transcriptase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:022266819</ref><ref group="xtra">PMID:019812032</ref><ref group="xtra">PMID:020852643</ref><ref group="xtra">PMID:018230722</ref><ref group="xtra">PMID:015115397</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Human immunodeficiency virus type 1 bh10]]
[[Category: Human immunodeficiency virus type 1 bh10]]
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[[Category: Arnold, E.]]
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[[Category: Arnold, E]]
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[[Category: Das, K.]]
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[[Category: Das, K]]
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[[Category: Martinez, S E.]]
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[[Category: Martinez, S E]]
[[Category: Aid]]
[[Category: Aid]]
[[Category: Dna recombination]]
[[Category: Dna recombination]]

Revision as of 17:44, 9 December 2014

Crystal structure of HIV-1 reverse transcriptase (RT) cross-linked with AZT-terminated DNA

3v6d, resolution 2.70Å

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