1nzv
From Proteopedia
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| - | [[Image:1nzv.jpg|left|200px]] | + | [[Image:1nzv.jpg|left|200px]] |
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| - | '''Crystal Structure of Src SH2 domain bound to doubly phosphorylated peptide PQpYIpYVPA''' | + | {{Structure |
| + | |PDB= 1nzv |SIZE=350|CAPTION= <scene name='initialview01'>1nzv</scene>, resolution 2.10Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=PG4:TETRAETHYLENE GLYCOL'>PG4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] | ||
| + | |GENE= V-SRC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11886 Rous sarcoma virus]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Src SH2 domain bound to doubly phosphorylated peptide PQpYIpYVPA''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NZV is a [ | + | 1NZV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZV OCA]. |
==Reference== | ==Reference== | ||
| - | Structural and thermodynamic basis for the interaction of the Src SH2 domain with the activated form of the PDGF beta-receptor., Lubman OY, Waksman G, J Mol Biol. 2003 May 2;328(3):655-68. PMID:[http:// | + | Structural and thermodynamic basis for the interaction of the Src SH2 domain with the activated form of the PDGF beta-receptor., Lubman OY, Waksman G, J Mol Biol. 2003 May 2;328(3):655-68. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12706723 12706723] |
[[Category: Rous sarcoma virus]] | [[Category: Rous sarcoma virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sh2 domain]] | [[Category: sh2 domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:03:53 2008'' |
Revision as of 11:03, 20 March 2008
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| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | V-SRC (Rous sarcoma virus) | ||||||
| Activity: | Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Src SH2 domain bound to doubly phosphorylated peptide PQpYIpYVPA
Overview
Recruitment of the Src kinase to the activated form of the platelet-derived growth factor (PDGF) receptor involves recognition of a unique sequence motif in the juxtamembrane region of the receptor by the Src homology 2 (SH2) domain of the enzyme. This motif contains two phosphotyrosine residues separated by one residue (sequence pYIpYV where pY indicates a phosphotyrosine). Here, we provide the thermodynamic and structural basis for the binding of this motif by the Src SH2 domain. We show that the second phosphorylation event increases the free energy window for specific interaction and that the physiological target is exquisitely designed for the task of recruiting specifically an SH2 domain which otherwise demonstrates very little intrinsic ability to discriminate sequences C-terminal to the first phosphorylation event. Surprisingly, we show that water plays a role in the recognition process.
About this Structure
1NZV is a Single protein structure of sequence from Rous sarcoma virus. Full crystallographic information is available from OCA.
Reference
Structural and thermodynamic basis for the interaction of the Src SH2 domain with the activated form of the PDGF beta-receptor., Lubman OY, Waksman G, J Mol Biol. 2003 May 2;328(3):655-68. PMID:12706723
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