1o08
From Proteopedia
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| - | [[Image:1o08.gif|left|200px]] | + | [[Image:1o08.gif|left|200px]] |
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| - | '''Structure of Pentavalent Phosphorous Intermediate of an Enzyme Catalyzed Phosphoryl transfer Reaction observed on cocrystallization with Glucose 1-phosphate''' | + | {{Structure |
| + | |PDB= 1o08 |SIZE=350|CAPTION= <scene name='initialview01'>1o08</scene>, resolution 1.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=G16:ALPHA-D-GLUCOSE+1,6-BISPHOSPHATE'>G16</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Beta-phosphoglucomutase Beta-phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.6 5.4.2.6] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structure of Pentavalent Phosphorous Intermediate of an Enzyme Catalyzed Phosphoryl transfer Reaction observed on cocrystallization with Glucose 1-phosphate''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1O08 is a [ | + | 1O08 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O08 OCA]. |
==Reference== | ==Reference== | ||
| - | The pentacovalent phosphorus intermediate of a phosphoryl transfer reaction., Lahiri SD, Zhang G, Dunaway-Mariano D, Allen KN, Science. 2003 Mar 28;299(5615):2067-71. Epub 2003 Mar 13. PMID:[http:// | + | The pentacovalent phosphorus intermediate of a phosphoryl transfer reaction., Lahiri SD, Zhang G, Dunaway-Mariano D, Allen KN, Science. 2003 Mar 28;299(5615):2067-71. Epub 2003 Mar 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12637673 12637673] |
[[Category: Beta-phosphoglucomutase]] | [[Category: Beta-phosphoglucomutase]] | ||
[[Category: Lactococcus lactis]] | [[Category: Lactococcus lactis]] | ||
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[[Category: phosphotransferase]] | [[Category: phosphotransferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:04:00 2008'' |
Revision as of 11:04, 20 March 2008
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| , resolution 1.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Beta-phosphoglucomutase, with EC number 5.4.2.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Pentavalent Phosphorous Intermediate of an Enzyme Catalyzed Phosphoryl transfer Reaction observed on cocrystallization with Glucose 1-phosphate
Overview
Enzymes provide enormous rate enhancements, unmatched by any other type of catalyst. The stabilization of high-energy states along the reaction coordinate is the crux of the catalytic power of enzymes. We report the atomic-resolution structure of a high-energy reaction intermediate stabilized in the active site of an enzyme. Crystallization of phosphorylated beta-phosphoglucomutase in the presence of the Mg(II) cofactor and either of the substrates glucose 1-phosphate or glucose 6-phosphate produced crystals of the enzyme-Mg(II)-glucose 1,6-(bis)phosphate complex, which diffracted x-rays to 1.2 and 1.4 angstroms, respectively. The structure reveals a stabilized pentacovalent phosphorane formed in the phosphoryl transfer from the C(1)O of glucose 1,6-(bis)phosphate to the nucleophilic Asp8 carboxylate.
About this Structure
1O08 is a Single protein structure of sequence from Lactococcus lactis. Full crystallographic information is available from OCA.
Reference
The pentacovalent phosphorus intermediate of a phosphoryl transfer reaction., Lahiri SD, Zhang G, Dunaway-Mariano D, Allen KN, Science. 2003 Mar 28;299(5615):2067-71. Epub 2003 Mar 13. PMID:12637673
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