1vs3

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[[Image:1vs3.png|left|200px]]
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==Crystal Structure of the tRNA Pseudouridine Synthase TruA From Thermus thermophilus HB8==
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<StructureSection load='1vs3' size='340' side='right' caption='[[1vs3]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1vs3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VS3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VS3 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">truA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA-pseudouridine_synthase_I tRNA-pseudouridine synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.12 5.4.99.12] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vs3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vs3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vs3 RCSB], [http://www.ebi.ac.uk/pdbsum/1vs3 PDBsum], [http://www.topsan.org/Proteins/RSGI/1vs3 TOPSAN]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vs/1vs3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The pseudouridine synthase (Psi synthase) TruA catalyzes the conversion of uridine to pseudouridine at positions 38, 39 and/or 40 in the anticodon stem-loop (ASL) of tRNA. We have determined the crystal structure of TruA from Thermus thermophilus HB8 at 2.25 A resolution. TruA and the other (Psi synthases have a completely conserved active site aspartate, which suggests that the members of this enzyme family share a common catalytic mechanism. The T. thermophilus TruA structure reveals the remarkably flexible structural features in the tRNA-binding cleft, which may be responsible for the primary tRNA interaction. In addition, the charged residues occupying the intermediate positions in the cleft may lead the tRNA to the active site for catalysis. Based on the TruB-tRNA complex structure, the T. thermophilus TruA structure reveals that the tRNA probably makes the melting base pairs move into the cleft, and suggests that a conformational change of the substrate tRNA is necessary to facilitate access to the active site aspartate residue, deep within the cleft.
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{{STRUCTURE_1vs3| PDB=1vs3 | SCENE= }}
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Crystal structure of tRNA pseudouridine synthase TruA from Thermus thermophilus HB8.,Dong X, Bessho Y, Shibata R, Nishimoto M, Shirouzu M, Kuramitsu S, Yokoyama S RNA Biol. 2006 Jul;3(3):115-22. Epub 2006 Jul 17. PMID:17114947<ref>PMID:17114947</ref>
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===Crystal Structure of the tRNA Pseudouridine Synthase TruA From Thermus thermophilus HB8===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_17114947}}
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==About this Structure==
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[[1vs3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VS3 OCA].
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==See Also==
==See Also==
*[[TRNA pseudouridine synthase|TRNA pseudouridine synthase]]
*[[TRNA pseudouridine synthase|TRNA pseudouridine synthase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:017114947</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
[[Category: TRNA-pseudouridine synthase I]]
[[Category: TRNA-pseudouridine synthase I]]

Revision as of 22:31, 28 September 2014

Crystal Structure of the tRNA Pseudouridine Synthase TruA From Thermus thermophilus HB8

1vs3, resolution 2.25Å

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