We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

301d

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:301d.png|left|200px]]
+
==CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED==
 +
<StructureSection load='301d' size='340' side='right' caption='[[301d]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[301d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=301D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=301D FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=301d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=301d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=301d RCSB], [http://www.ebi.ac.uk/pdbsum/301d PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The crystal structure of an unmodified hammerhead RNA in the absence of divalent metal ions has been solved, and it was shown that this ribozyme can cleave itself in the crystal when divalent metal ions are added. This biologically active RNA fold is the same as that found previously for two modified hammerhead ribozymes. Addition of divalent cations at low pH makes it possible to capture the uncleaved RNA in metal-bound form. A conformational intermediate, having an additional Mg(II) bound to the cleavage-site phosphate, was captured by freeze-trapping the RNA at an active pH prior to cleavage. The most significant conformational changes were limited to the active site of the ribozyme, and the changed conformation requires only small additional movements to reach a proposed transition-state.
-
{{STRUCTURE_301d| PDB=301d | SCENE= }}
+
Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme.,Scott WG, Murray JB, Arnold JR, Stoddard BL, Klug A Science. 1996 Dec 20;274(5295):2065-9. PMID:8953035<ref>PMID:8953035</ref>
-
===CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_8953035}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
[[301d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=301D OCA].
+
</StructureSection>
-
 
+
-
==See Also==
+
-
*[[Ribozyme|Ribozyme]]
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:008953035</ref><ref group="xtra">PMID:011779233</ref><references group="xtra"/>
+
[[Category: Arnold, J R.P.]]
[[Category: Arnold, J R.P.]]
[[Category: Klug, A.]]
[[Category: Klug, A.]]

Revision as of 11:49, 29 October 2014

CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED

301d, resolution 3.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/301d"
Personal tools