4efh

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[[Image:4efh.png|left|200px]]
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==Acanthamoeba Actin complex with Spir domain D==
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<StructureSection load='4efh' size='340' side='right' caption='[[4efh]], [[Resolution|resolution]] 2.48&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4efh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Acanthamoeba_castellanii Acanthamoeba castellanii] and [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EFH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EFH FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ue5|3ue5]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spir, p150-Spir, CG10076 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4efh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4efh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4efh RCSB], [http://www.ebi.ac.uk/pdbsum/4efh PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/SPIR_DROME SPIR_DROME]] Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Promotes dissociation of capu from the barbed end of actin filaments. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for localization of determinants within the developing oocyte to the posterior pole and to the dorsal anterior corner. Links Rho family signaling and Jnk function to the actin cytoskeleton.<ref>PMID:10556052</ref> <ref>PMID:10744979</ref> <ref>PMID:15674283</ref> <ref>PMID:20538977</ref> <ref>PMID:21730168</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Spire is a WH2 domain-containing actin nucleator essential for establishing an actin mesh during oogenesis. In vitro, in addition to nucleating filaments, Spire can sever them and sequester actin monomers. Understanding how Spire is capable of these disparate functions and which are physiologically relevant is an important goal. To study severing, we examined the effect of Drosophila Spire on preformed filaments in bulk and single filament assays. We observed rapid depolymerization of actin filaments by Spire, which we conclude is largely due to its sequestration activity and enhanced by its weak severing activity. We also studied the solution and crystal structures of Spire-actin complexes. We find structural and functional differences between constructs containing four WH2-domains (Spir-ABCD) and two WH2-domains (Spir-CD) that may provide insight into mechanisms of nucleation and sequestration. Intriguingly, we observed lateral interactions between actin monomers associated with Spir-ABCD, suggesting that the structures built by these four tandem WH2 domains are more complex than originally imagined. Finally, we propose that Spire-actin mixtures contain both nuclei and sequestration structures.
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{{STRUCTURE_4efh| PDB=4efh | SCENE= }}
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Multiple forms of Spire-actin complexes and their functional consequences.,Chen CK, Sawaya MR, Phillips ML, Reisler E, Quinlan ME J Biol Chem. 2012 Feb 8. PMID:22334675<ref>PMID:22334675</ref>
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===Acanthamoeba Actin complex with Spir domain D===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_22334675}}
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==About this Structure==
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[[4efh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Acanthamoeba_castellanii Acanthamoeba castellanii] and [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EFH OCA].
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==See Also==
==See Also==
*[[Actin|Actin]]
*[[Actin|Actin]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:022334675</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Acanthamoeba castellanii]]
[[Category: Acanthamoeba castellanii]]
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Chen, C.]]
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[[Category: Chen, C]]
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[[Category: Phillips, M.]]
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[[Category: Phillips, M]]
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[[Category: Quinlan, M E.]]
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[[Category: Quinlan, M E]]
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[[Category: Sawaya, M R.]]
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[[Category: Sawaya, M R]]
[[Category: Contractile protein]]
[[Category: Contractile protein]]
[[Category: Contractile protein-transport protein complex]]
[[Category: Contractile protein-transport protein complex]]
[[Category: Cytoskeleton]]
[[Category: Cytoskeleton]]

Revision as of 21:43, 25 December 2014

Acanthamoeba Actin complex with Spir domain D

4efh, resolution 2.48Å

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