1o76

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[[Image:1o76.jpg|left|200px]]<br /><applet load="1o76" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1o76.jpg|left|200px]]
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caption="1o76, resolution 1.8&Aring;" />
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'''CYANIDE COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA'''<br />
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{{Structure
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|PDB= 1o76 |SIZE=350|CAPTION= <scene name='initialview01'>1o76</scene>, resolution 1.8&Aring;
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|SITE= <scene name='pdbsite=AC1:K+Binding+Site+For+Chain+B'>AC1</scene>
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|LIGAND= <scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=CAM:CAMPHOR'>CAM</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1]
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|GENE=
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}}
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'''CYANIDE COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1O76 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with <scene name='pdbligand=CYN:'>CYN</scene>, <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=CAM:'>CAM</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Camphor_5-monooxygenase Camphor 5-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.15.1 1.14.15.1] Known structural/functional Site: <scene name='pdbsite=AC1:K+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O76 OCA].
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1O76 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O76 OCA].
==Reference==
==Reference==
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Crystal structures of cyanide complexes of P450cam and the oxygenase domain of inducible nitric oxide synthase-structural models of the short-lived oxygen complexes., Fedorov R, Ghosh DK, Schlichting I, Arch Biochem Biophys. 2003 Jan 1;409(1):25-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12464241 12464241]
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Crystal structures of cyanide complexes of P450cam and the oxygenase domain of inducible nitric oxide synthase-structural models of the short-lived oxygen complexes., Fedorov R, Ghosh DK, Schlichting I, Arch Biochem Biophys. 2003 Jan 1;409(1):25-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12464241 12464241]
[[Category: Camphor 5-monooxygenase]]
[[Category: Camphor 5-monooxygenase]]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
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[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:14:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:06:40 2008''

Revision as of 11:06, 20 March 2008

Image:1o76.jpg


PDB ID 1o76

Drag the structure with the mouse to rotate
, resolution 1.8Å
Sites:
Ligands: , , , and
Activity: Camphor 5-monooxygenase, with EC number 1.14.15.1
Coordinates: save as pdb, mmCIF, xml



CYANIDE COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA


Overview

The crystal structure of the ternary cyanide complex of P450cam and camphor was determined to 1.8A resolution and found to be identical with the structure of the active oxygen complex [I. Schlichting et al., 2000, Science 287, 1615]. Notably, cyanide binds in a bent mode and induces the active conformation that is characterized by the presence of two water molecules and a flip of the carbonyl of the conserved Asp251. The structure of the ternary complex of cyanide, L-arginine, and the oxygenase domain of inducible nitric oxide synthase was determined to 2.4A resolution. Cyanide binds essentially linearly, interacts with L-Arg, and induces the binding of a water molecule at the active site. This water is positioned by backbone interactions, located 2.8A from the nitrogen atom of cyanide, and could provide a proton required for O-O bond scission in the hydroxylation reaction of nitric oxide synthase.

About this Structure

1O76 is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Crystal structures of cyanide complexes of P450cam and the oxygenase domain of inducible nitric oxide synthase-structural models of the short-lived oxygen complexes., Fedorov R, Ghosh DK, Schlichting I, Arch Biochem Biophys. 2003 Jan 1;409(1):25-31. PMID:12464241

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