2ex5

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[[Image:2ex5.png|left|200px]]
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==Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA==
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<StructureSection load='2ex5' size='340' side='right' caption='[[2ex5]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ex5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_moewusii Chlamydomonas moewusii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EX5 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1af5|1af5]], [[1bp7|1bp7]], [[1g9y|1g9y]], [[1g9z|1g9z]], [[1m5x|1m5x]], [[1mow|1mow]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">I-CeuI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3054 Chlamydomonas moewusii])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ex5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ex5 RCSB], [http://www.ebi.ac.uk/pdbsum/2ex5 PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/2ex5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Homing endonucleases are highly specific catalysts of DNA strand breaks, leading to the transfer of mobile intervening sequences containing the endonuclease ORF. We have determined the structure and DNA recognition behavior of I-CeuI, a homodimeric LAGLIDADG endonuclease from Chlamydomonas eugametos. This symmetric endonuclease displays unique structural elaborations on its core enzyme fold, and it preferentially cleaves a highly asymmetric target site. This latter property represents an early step, prior to gene fusion, in the generation of asymmetric DNA binding platforms from homodimeric ancestors. The divergence of the sequence, structure, and target recognition behavior of homing endonucleases, as illustrated by this study, leads to the invasion of novel genomic sites by mobile introns during evolution.
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{{STRUCTURE_2ex5| PDB=2ex5 | SCENE= }}
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The structure of I-CeuI homing endonuclease: Evolving asymmetric DNA recognition from a symmetric protein scaffold.,Spiegel PC, Chevalier B, Sussman D, Turmel M, Lemieux C, Stoddard BL Structure. 2006 May;14(5):869-80. PMID:16698548<ref>PMID:16698548</ref>
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===Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_16698548}}
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==About this Structure==
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[[2ex5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_moewusii Chlamydomonas moewusii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX5 OCA].
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==See Also==
==See Also==
*[[Endonuclease|Endonuclease]]
*[[Endonuclease|Endonuclease]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016698548</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Chlamydomonas moewusii]]
[[Category: Chlamydomonas moewusii]]
[[Category: Spiegel, P C.]]
[[Category: Spiegel, P C.]]

Revision as of 06:43, 10 October 2014

Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA

2ex5, resolution 2.20Å

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