1ooa
From Proteopedia
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| - | [[Image:1ooa.gif|left|200px]] | + | [[Image:1ooa.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF NF-kB(p50)2 COMPLEXED TO A HIGH-AFFINITY RNA APTAMER''' | + | {{Structure |
| + | |PDB= 1ooa |SIZE=350|CAPTION= <scene name='initialview01'>1ooa</scene>, resolution 2.45Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= NFKB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF NF-kB(p50)2 COMPLEXED TO A HIGH-AFFINITY RNA APTAMER''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OOA is a [ | + | 1OOA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OOA OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of NF-kappaB (p50)2 complexed to a high-affinity RNA aptamer., Huang DB, Vu D, Cassiday LA, Zimmerman JM, Maher LJ 3rd, Ghosh G, Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9268-73. Epub 2003 Jul 28. PMID:[http:// | + | Crystal structure of NF-kappaB (p50)2 complexed to a high-affinity RNA aptamer., Huang DB, Vu D, Cassiday LA, Zimmerman JM, Maher LJ 3rd, Ghosh G, Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9268-73. Epub 2003 Jul 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12886018 12886018] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transcription factor nf-kb]] | [[Category: transcription factor nf-kb]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:13:32 2008'' |
Revision as of 11:13, 20 March 2008
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| , resolution 2.45Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | NFKB1 (Mus musculus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF NF-kB(p50)2 COMPLEXED TO A HIGH-AFFINITY RNA APTAMER
Overview
We have recently identified an RNA aptamer for the transcription factor NF-kappaB p50 homodimer [(p50)2], which exhibits little sequence resemblance to the consensus DNA target for (p50)2, but binds (p50)2 with an affinity similar to that of the optimal DNA target. We describe here the 2.45-A resolution x-ray crystal structure of the p50 RHR/RNA aptamer complex. The structure reveals that two RNA molecules bind independent of each other to the p50 N-terminal Ig-like domains. The RNA secondary structure is comprised of a stem and a stem-loop separated by an internal loop folded into a kinked helix because of the cross-strand stacking of three internal loop guanines. These guanines, placed at the edge of the 3' helix, together with the major groove of the irregular 3' helix, form the binding surface for p50. Each p50 monomer uses the same surface to recognize the distorted RNA major groove as observed in the kappaB DNA/p50 RHR complex, resulting in strikingly similar interfaces. The structure reveals how the aptamer specifically selects p50 and discriminates against p65. We also discuss the physiological implications of RNA binding by (p50)2.
About this Structure
1OOA is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structure of NF-kappaB (p50)2 complexed to a high-affinity RNA aptamer., Huang DB, Vu D, Cassiday LA, Zimmerman JM, Maher LJ 3rd, Ghosh G, Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9268-73. Epub 2003 Jul 28. PMID:12886018
Page seeded by OCA on Thu Mar 20 13:13:32 2008
