1orr
From Proteopedia
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| - | [[Image:1orr.jpg|left|200px]] | + | [[Image:1orr.jpg|left|200px]] |
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| - | '''Crystal Structure of CDP-Tyvelose 2-Epimerase complexed with NAD and CDP''' | + | {{Structure |
| + | |PDB= 1orr |SIZE=350|CAPTION= <scene name='initialview01'>1orr</scene>, resolution 1.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> and <scene name='pdbligand=CDP:CYTIDINE-5'-DIPHOSPHATE'>CDP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= RFBE OR STY2298 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=601 Salmonella typhi]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of CDP-Tyvelose 2-Epimerase complexed with NAD and CDP''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ORR is a [ | + | 1ORR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhi Salmonella typhi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORR OCA]. |
==Reference== | ==Reference== | ||
| - | High resolution x-ray structure of tyvelose epimerase from Salmonella typhi., Koropatkin NM, Liu HW, Holden HM, J Biol Chem. 2003 Jun 6;278(23):20874-81. Epub 2003 Mar 17. PMID:[http:// | + | High resolution x-ray structure of tyvelose epimerase from Salmonella typhi., Koropatkin NM, Liu HW, Holden HM, J Biol Chem. 2003 Jun 6;278(23):20874-81. Epub 2003 Mar 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12642575 12642575] |
[[Category: Salmonella typhi]] | [[Category: Salmonella typhi]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: short-chain dehydrogenase/reductase]] | [[Category: short-chain dehydrogenase/reductase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:14:44 2008'' |
Revision as of 11:14, 20 March 2008
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| , resolution 1.50Å | |||||||
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| Ligands: | and | ||||||
| Gene: | RFBE OR STY2298 (Salmonella typhi) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of CDP-Tyvelose 2-Epimerase complexed with NAD and CDP
Overview
Tyvelose epimerase catalyzes the last step in the biosynthesis of tyvelose by converting CDP-d-paratose to CDP-d-tyvelose. This unusual 3,6-dideoxyhexose occurs in the O-antigens of some types of Gram-negative bacteria. Here we describe the cloning, protein purification, and high-resolution x-ray crystallographic analysis of tyvelose epimerase from Salmonella typhi complexed with CDP. The enzyme from S. typhi is a homotetramer with each subunit containing 339 amino acid residues and a tightly bound NAD+ cofactor. The quaternary structure of the enzyme displays 222 symmetry and can be aptly described as a dimer of dimers. Each subunit folds into two distinct lobes: the N-terminal motif responsible for NAD+ binding and the C-terminal region that harbors the binding site for CDP. The analysis described here demonstrates that tyvelose epimerase belongs to the short-chain dehydrogenase/reductase superfamily of enzymes. Indeed, its active site is reminiscent to that observed for UDP-galactose 4-epimerase, an enzyme that plays a key role in galactose metabolism. Unlike UDP-galactose 4-epimerase where the conversion of configuration occurs about C-4 of the UDP-glucose or UDP-galactose substrates, in the reaction catalyzed by tyvelose epimerase, the inversion of stereochemistry occurs at C-2. On the basis of the observed binding mode for CDP, it is possible to predict the manner in which the substrate, CDP-paratose, and the product, CDP-tyvelose, might be accommodated within the active site of tyvelose epimerase.
About this Structure
1ORR is a Single protein structure of sequence from Salmonella typhi. Full crystallographic information is available from OCA.
Reference
High resolution x-ray structure of tyvelose epimerase from Salmonella typhi., Koropatkin NM, Liu HW, Holden HM, J Biol Chem. 2003 Jun 6;278(23):20874-81. Epub 2003 Mar 17. PMID:12642575
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