1owf
From Proteopedia
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| - | [[Image:1owf.gif|left|200px]] | + | [[Image:1owf.gif|left|200px]] |
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| - | '''Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site''' | + | {{Structure |
| + | |PDB= 1owf |SIZE=350|CAPTION= <scene name='initialview01'>1owf</scene>, resolution 1.95Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OWF is a [ | + | 1OWF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OWF OCA]. |
==Reference== | ==Reference== | ||
| - | Integration host factor: putting a twist on protein-DNA recognition., Lynch TW, Read EK, Mattis AN, Gardner JF, Rice PA, J Mol Biol. 2003 Jul 11;330(3):493-502. PMID:[http:// | + | Integration host factor: putting a twist on protein-DNA recognition., Lynch TW, Read EK, Mattis AN, Gardner JF, Rice PA, J Mol Biol. 2003 Jul 11;330(3):493-502. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12842466 12842466] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: protein-dna recognition]] | [[Category: protein-dna recognition]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:16:33 2008'' |
Revision as of 11:16, 20 March 2008
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| , resolution 1.95Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a mutant IHF (BetaE44A) complexed with the native H' Site
Overview
Integration host factor (IHF) is a DNA-bending protein that recognizes its cognate sites through indirect readout. Previous studies have shown that binding of wild-type (WT)-IHF is disrupted by a T to A mutation at the center position of a conserved TTR motif in its binding site, and that substitution of betaGlu44 with Ala prevented IHF from discriminating between A and T at this position. We have determined the crystal structures and relative binding affinities for all combinations of WT-IHF and IHF-betaGlu44Ala bound to the WT and mutant DNAs. Comparison of these structures reveals that DNA twist plays a major role in DNA recognition by IHF, and that this geometric parameter is dependent on the dinucleotide step and not on the bound IHF variant.
About this Structure
1OWF is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Integration host factor: putting a twist on protein-DNA recognition., Lynch TW, Read EK, Mattis AN, Gardner JF, Rice PA, J Mol Biol. 2003 Jul 11;330(3):493-502. PMID:12842466
Page seeded by OCA on Thu Mar 20 13:16:33 2008
