1oxj
From Proteopedia
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| - | [[Image:1oxj.jpg|left|200px]] | + | [[Image:1oxj.jpg|left|200px]] |
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| - | '''Crystal structure of the Smaug RNA binding domain''' | + | {{Structure |
| + | |PDB= 1oxj |SIZE=350|CAPTION= <scene name='initialview01'>1oxj</scene>, resolution 1.8Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= SMG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the Smaug RNA binding domain''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OXJ is a [ | + | 1OXJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXJ OCA]. |
==Reference== | ==Reference== | ||
| - | RNA recognition via the SAM domain of Smaug., Green JB, Gardner CD, Wharton RP, Aggarwal AK, Mol Cell. 2003 Jun;11(6):1537-48. PMID:[http:// | + | RNA recognition via the SAM domain of Smaug., Green JB, Gardner CD, Wharton RP, Aggarwal AK, Mol Cell. 2003 Jun;11(6):1537-48. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12820967 12820967] |
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sam domain]] | [[Category: sam domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:16:59 2008'' |
Revision as of 11:17, 20 March 2008
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| , resolution 1.8Å | |||||||
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| Gene: | SMG (Drosophila melanogaster) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Smaug RNA binding domain
Overview
The Nanos protein gradient in Drosophila, required for proper abdominal segmentation, is generated in part via translational repression of its mRNA by Smaug. We report here the crystal structure of the Smaug RNA binding domain, which shows no sequence homology to any previously characterized RNA binding motif. The structure reveals an unusual makeup in which a SAM domain, a common protein-protein interaction module, is affixed to a pseudo-HEAT repeat analogous topology (PHAT) domain. Unexpectedly, we find through a combination of structural and genetic analysis that it is primarily the SAM domain that interacts specifically with the appropriate nanos mRNA regulatory sequence. Therefore, in addition to their previously characterized roles in protein-protein interactions, some SAM domains play crucial roles in RNA binding.
About this Structure
1OXJ is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
RNA recognition via the SAM domain of Smaug., Green JB, Gardner CD, Wharton RP, Aggarwal AK, Mol Cell. 2003 Jun;11(6):1537-48. PMID:12820967
Page seeded by OCA on Thu Mar 20 13:16:59 2008
