1oys
From Proteopedia
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| - | [[Image:1oys.jpg|left|200px]] | + | [[Image:1oys.jpg|left|200px]] |
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| - | '''Crystal Structure of the Phosphorolytic Exoribonuclease RNase PH from Bacillus subtilis''' | + | {{Structure |
| + | |PDB= 1oys |SIZE=350|CAPTION= <scene name='initialview01'>1oys</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/tRNA_nucleotidyltransferase tRNA nucleotidyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.56 2.7.7.56] | ||
| + | |GENE= RPH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Phosphorolytic Exoribonuclease RNase PH from Bacillus subtilis''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OYS is a [ | + | 1OYS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OYS OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis and implications for its quaternary structure and tRNA binding., Harlow LS, Kadziola A, Jensen KF, Larsen S, Protein Sci. 2004 Mar;13(3):668-77. Epub 2004 Feb 6. PMID:[http:// | + | Crystal structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis and implications for its quaternary structure and tRNA binding., Harlow LS, Kadziola A, Jensen KF, Larsen S, Protein Sci. 2004 Mar;13(3):668-77. Epub 2004 Feb 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14767080 14767080] |
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: trna processing]] | [[Category: trna processing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:17:29 2008'' |
Revision as of 11:17, 20 March 2008
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| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | RPH (Bacillus subtilis) | ||||||
| Activity: | tRNA nucleotidyltransferase, with EC number 2.7.7.56 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Phosphorolytic Exoribonuclease RNase PH from Bacillus subtilis
Overview
RNase PH is a member of the family of phosphorolytic 3' --> 5' exoribonucleases that also includes polynucleotide phosphorylase (PNPase). RNase PH is involved in the maturation of tRNA precursors and especially important for removal of nucleotide residues near the CCA acceptor end of the mature tRNAs. Wild-type and triple mutant R68Q-R73Q-R76Q RNase PH from Bacillus subtilis have been crystallized and the structures determined by X-ray diffraction to medium resolution. Wild-type and triple mutant RNase PH crystallize as a hexamer and dimer, respectively. The structures contain a rare left-handed beta alpha beta-motif in the N-terminal portion of the protein. This motif has also been identified in other enzymes involved in RNA metabolism. The RNase PH structure and active site can, despite low sequence similarity, be overlayed with the N-terminal core of the structure and active site of Streptomyces antibioticus PNPase. The surface of the RNase PH dimer fit the shape of a tRNA molecule.
About this Structure
1OYS is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Crystal structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis and implications for its quaternary structure and tRNA binding., Harlow LS, Kadziola A, Jensen KF, Larsen S, Protein Sci. 2004 Mar;13(3):668-77. Epub 2004 Feb 6. PMID:14767080
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