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1oyu
From Proteopedia
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| - | [[Image:1oyu.jpg|left|200px]] | + | [[Image:1oyu.jpg|left|200px]] |
| - | + | ||
| - | '''Long-Distance conformational changes in a protein engineered by modulated sequence duplication''' | + | {{Structure |
| + | |PDB= 1oyu |SIZE=350|CAPTION= <scene name='initialview01'>1oyu</scene>, resolution 2.5Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Long-Distance conformational changes in a protein engineered by modulated sequence duplication''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1OYU is a [ | + | 1OYU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OYU OCA]. |
==Reference== | ==Reference== | ||
| - | Long-distance conformational changes in a protein engineered by modulated sequence duplication., Sagermann M, Gay L, Matthews BW, Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9191-5. Epub 2003 Jul 17. PMID:[http:// | + | Long-distance conformational changes in a protein engineered by modulated sequence duplication., Sagermann M, Gay L, Matthews BW, Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9191-5. Epub 2003 Jul 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12869697 12869697] |
[[Category: Bacteriophage t4]] | [[Category: Bacteriophage t4]] | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
| Line 17: | Line 26: | ||
[[Category: Matthews, B W.]] | [[Category: Matthews, B W.]] | ||
[[Category: Sagermann, M.]] | [[Category: Sagermann, M.]] | ||
| - | [[Category: design of structural | + | [[Category: design of structural switch]] |
[[Category: protein design]] | [[Category: protein design]] | ||
[[Category: sequence duplication]] | [[Category: sequence duplication]] | ||
[[Category: tandem repeat]] | [[Category: tandem repeat]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:17:35 2008'' |
Revision as of 11:17, 20 March 2008
| |||||||
| , resolution 2.5Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Long-Distance conformational changes in a protein engineered by modulated sequence duplication
Overview
There are few, if any, known instances in which a biological signal is transmitted via a large conformational change through the body of a protein. We describe here a mutant of T4 lysozyme that was engineered to permit structural change at a distance. The design uses a tandem sequence repeat that makes it possible to transmit large-scale structural changes from one end of an alpha-helix to the other over a distance of 17-25 A. The method should be of general applicability and may make it possible to introduce a mutation at one site in a protein that will induce large-scale changes in the structure at a spatially remote site.
About this Structure
1OYU is a Single protein structure of sequence from Bacteriophage t4. Full crystallographic information is available from OCA.
Reference
Long-distance conformational changes in a protein engineered by modulated sequence duplication., Sagermann M, Gay L, Matthews BW, Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9191-5. Epub 2003 Jul 17. PMID:12869697
Page seeded by OCA on Thu Mar 20 13:17:35 2008
