1p0c

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[[Image:1p0c.jpg|left|200px]]<br /><applet load="1p0c" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1p0c.jpg|left|200px]]
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caption="1p0c, resolution 2.2&Aring;" />
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'''Crystal Structure of the NADP(H)-Dependent Vertebrate Alcohol Dehydrogenase (ADH8)'''<br />
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{{Structure
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|PDB= 1p0c |SIZE=350|CAPTION= <scene name='initialview01'>1p0c</scene>, resolution 2.2&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(NADP(+)) Alcohol dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.2 1.1.1.2]
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|GENE= ADH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=88445 Rana porosa])
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}}
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'''Crystal Structure of the NADP(H)-Dependent Vertebrate Alcohol Dehydrogenase (ADH8)'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1P0C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rana_porosa Rana porosa] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(NADP(+)) Alcohol dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.2 1.1.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P0C OCA].
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1P0C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rana_porosa Rana porosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P0C OCA].
==Reference==
==Reference==
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Crystal structure of the vertebrate NADP(H)-dependent alcohol dehydrogenase (ADH8)., Rosell A, Valencia E, Pares X, Fita I, Farres J, Ochoa WF, J Mol Biol. 2003 Jun 27;330(1):75-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12818203 12818203]
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Crystal structure of the vertebrate NADP(H)-dependent alcohol dehydrogenase (ADH8)., Rosell A, Valencia E, Pares X, Fita I, Farres J, Ochoa WF, J Mol Biol. 2003 Jun 27;330(1):75-85. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12818203 12818203]
[[Category: Alcohol dehydrogenase (NADP(+))]]
[[Category: Alcohol dehydrogenase (NADP(+))]]
[[Category: Rana porosa]]
[[Category: Rana porosa]]
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[[Category: nadp(h)-dependent]]
[[Category: nadp(h)-dependent]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:23:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:18:04 2008''

Revision as of 11:18, 20 March 2008

Image:1p0c.jpg


PDB ID 1p0c

Drag the structure with the mouse to rotate
, resolution 2.2Å
Ligands: , and
Gene: ADH (Rana porosa)
Activity: Alcohol dehydrogenase (NADP(+)), with EC number 1.1.1.2
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of the NADP(H)-Dependent Vertebrate Alcohol Dehydrogenase (ADH8)


Overview

The amphibian enzyme ADH8, previously named class IV-like, is the only known vertebrate alcohol dehydrogenase (ADH) with specificity towards NADP(H). The three-dimensional structures of ADH8 and of the binary complex ADH8-NADP(+) have been now determined and refined to resolutions of 2.2A and 1.8A, respectively. The coenzyme and substrate specificity of ADH8, that has 50-65% sequence identity with vertebrate NAD(H)-dependent ADHs, suggest a role in aldehyde reduction probably as a retinal reductase. The large volume of the substrate-binding pocket can explain both the high catalytic efficiency of ADH8 with retinoids and the high K(m) value for ethanol. Preference of NADP(H) appears to be achieved by the presence in ADH8 of the triad Gly223-Thr224-His225 and the recruitment of conserved Lys228, which define a binding pocket for the terminal phosphate group of the cofactor. NADP(H) binds to ADH8 in an extended conformation that superimposes well with the NAD(H) molecules found in NAD(H)-dependent ADH complexes. No additional reshaping of the dinucleotide-binding site is observed which explains why NAD(H) can also be used as a cofactor by ADH8. The structural features support the classification of ADH8 as an independent ADH class.

About this Structure

1P0C is a Single protein structure of sequence from Rana porosa. Full crystallographic information is available from OCA.

Reference

Crystal structure of the vertebrate NADP(H)-dependent alcohol dehydrogenase (ADH8)., Rosell A, Valencia E, Pares X, Fita I, Farres J, Ochoa WF, J Mol Biol. 2003 Jun 27;330(1):75-85. PMID:12818203

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