1p0s
From Proteopedia
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| - | [[Image:1p0s.jpg|left|200px]] | + | [[Image:1p0s.jpg|left|200px]] |
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| - | '''Crystal Structure of Blood Coagulation Factor Xa in Complex with Ecotin M84R''' | + | {{Structure |
| + | |PDB= 1p0s |SIZE=350|CAPTION= <scene name='initialview01'>1p0s</scene>, resolution 2.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Coagulation_factor_Xa Coagulation factor Xa], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.6 3.4.21.6] | ||
| + | |GENE= ECO OR ETI OR B2209 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Blood Coagulation Factor Xa in Complex with Ecotin M84R''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1P0S is a [ | + | 1P0S is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P0S OCA]. |
==Reference== | ==Reference== | ||
| - | The extended interactions and Gla domain of blood coagulation factor Xa., Wang SX, Hur E, Sousa CA, Brinen L, Slivka EJ, Fletterick RJ, Biochemistry. 2003 Jul 8;42(26):7959-66. PMID:[http:// | + | The extended interactions and Gla domain of blood coagulation factor Xa., Wang SX, Hur E, Sousa CA, Brinen L, Slivka EJ, Fletterick RJ, Biochemistry. 2003 Jul 8;42(26):7959-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12834348 12834348] |
[[Category: Coagulation factor Xa]] | [[Category: Coagulation factor Xa]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: serine protease inhibitor]] | [[Category: serine protease inhibitor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:18:18 2008'' |
Revision as of 11:18, 20 March 2008
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| , resolution 2.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | ECO OR ETI OR B2209 (Escherichia coli) | ||||||
| Activity: | Coagulation factor Xa, with EC number 3.4.21.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Blood Coagulation Factor Xa in Complex with Ecotin M84R
Contents |
Overview
The serine protease factor Xa (FXa) is inhibited by ecotin with picomolar affinity. The structure of the tetrameric complex of ecotin variant M84R (M84R) with FXa has been determined to 2.8 A. Substrate directed induced fit of the binding interactions at the S2 and S4 pockets modulates the discrimination of the protease. Specifically, the Tyr at position 99 of FXa changes its conformation with respect to incoming ligand, changing the size of the S2 and S4 pockets. The role of residue 192 in substrate and inhibitor recognition is also examined. Gln 192 from FXa forms a hydrogen bond with the P2 carbonyl group of ecotin. This confirms previous biochemical and structural analyses on thrombin and activated protein C, which suggested that residue 192 may play a more general role in mediating the interactions between coagulation proteases and their inhibitors. The structure of ecotin M84R-FXa (M84R-FXa) also reveals the structure of the Gla domain in the presence of Mg(2+). The first 11 residues of the domain assume a novel conformation and likely represent an intermediate folding state of the domain.
Disease
Known disease associated with this structure: Factor X deficiency OMIM:[227600]
About this Structure
1P0S is a Protein complex structure of sequences from Escherichia coli and Homo sapiens. Full crystallographic information is available from OCA.
Reference
The extended interactions and Gla domain of blood coagulation factor Xa., Wang SX, Hur E, Sousa CA, Brinen L, Slivka EJ, Fletterick RJ, Biochemistry. 2003 Jul 8;42(26):7959-66. PMID:12834348
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