1p5u
From Proteopedia
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| - | [[Image:1p5u.jpg|left|200px]] | + | [[Image:1p5u.jpg|left|200px]] |
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| - | '''X-ray structure of the ternary Caf1M:Caf1:Caf1 chaperone:subunit:subunit complex''' | + | {{Structure |
| + | |PDB= 1p5u |SIZE=350|CAPTION= <scene name='initialview01'>1p5u</scene>, resolution 1.99Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= CAF1M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 Yersinia pestis]), CAF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 Yersinia pestis]) | ||
| + | }} | ||
| + | |||
| + | '''X-ray structure of the ternary Caf1M:Caf1:Caf1 chaperone:subunit:subunit complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1P5U is a [ | + | 1P5U is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P5U OCA]. |
==Reference== | ==Reference== | ||
| - | Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation., Zavialov AV, Berglund J, Pudney AF, Fooks LJ, Ibrahim TM, MacIntyre S, Knight SD, Cell. 2003 May 30;113(5):587-96. PMID:[http:// | + | Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation., Zavialov AV, Berglund J, Pudney AF, Fooks LJ, Ibrahim TM, MacIntyre S, Knight SD, Cell. 2003 May 30;113(5):587-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12787500 12787500] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Yersinia pestis]] | [[Category: Yersinia pestis]] | ||
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[[Category: protein fiber]] | [[Category: protein fiber]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:20:13 2008'' |
Revision as of 11:20, 20 March 2008
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| , resolution 1.99Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | CAF1M (Yersinia pestis), CAF1 (Yersinia pestis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-ray structure of the ternary Caf1M:Caf1:Caf1 chaperone:subunit:subunit complex
Overview
Most gram-negative pathogens express fibrous adhesive virulence organelles that mediate targeting to the sites of infection. The F1 capsular antigen from the plague pathogen Yersinia pestis consists of linear fibers of a single subunit (Caf1) and serves as a prototype for nonpilus organelles assembled via the chaperone/usher pathway. Genetic data together with high-resolution X-ray structures corresponding to snapshots of the assembly process reveal the structural basis of fiber formation. Comparison of chaperone bound Caf1 subunit with the subunit in the fiber reveals a novel type of conformational change involving the entire hydrophobic core of the protein. The observed conformational change suggests that the chaperone traps a high-energy folding intermediate of Caf1. A model is proposed in which release of the subunit allows folding to be completed, driving fiber formation.
About this Structure
1P5U is a Protein complex structure of sequences from Yersinia pestis. Full crystallographic information is available from OCA.
Reference
Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation., Zavialov AV, Berglund J, Pudney AF, Fooks LJ, Ibrahim TM, MacIntyre S, Knight SD, Cell. 2003 May 30;113(5):587-96. PMID:12787500
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