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1p5v

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[[Image:1p5v.jpg|left|200px]]<br /><applet load="1p5v" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1p5v.jpg|left|200px]]
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caption="1p5v, resolution 1.70&Aring;" />
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'''X-ray structure of the Caf1M:Caf1 chaperone:subunit preassembly complex'''<br />
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{{Structure
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|PDB= 1p5v |SIZE=350|CAPTION= <scene name='initialview01'>1p5v</scene>, resolution 1.70&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE= CAF1M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 Yersinia pestis]), CAF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 Yersinia pestis])
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}}
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'''X-ray structure of the Caf1M:Caf1 chaperone:subunit preassembly complex'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1P5V is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P5V OCA].
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1P5V is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P5V OCA].
==Reference==
==Reference==
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Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation., Zavialov AV, Berglund J, Pudney AF, Fooks LJ, Ibrahim TM, MacIntyre S, Knight SD, Cell. 2003 May 30;113(5):587-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12787500 12787500]
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Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation., Zavialov AV, Berglund J, Pudney AF, Fooks LJ, Ibrahim TM, MacIntyre S, Knight SD, Cell. 2003 May 30;113(5):587-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12787500 12787500]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Yersinia pestis]]
[[Category: Yersinia pestis]]
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[[Category: protein fiber]]
[[Category: protein fiber]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:20:13 2008''

Revision as of 11:20, 20 March 2008


PDB ID 1p5v

Drag the structure with the mouse to rotate
, resolution 1.70Å
Gene: CAF1M (Yersinia pestis), CAF1 (Yersinia pestis)
Coordinates: save as pdb, mmCIF, xml



X-ray structure of the Caf1M:Caf1 chaperone:subunit preassembly complex


Overview

Most gram-negative pathogens express fibrous adhesive virulence organelles that mediate targeting to the sites of infection. The F1 capsular antigen from the plague pathogen Yersinia pestis consists of linear fibers of a single subunit (Caf1) and serves as a prototype for nonpilus organelles assembled via the chaperone/usher pathway. Genetic data together with high-resolution X-ray structures corresponding to snapshots of the assembly process reveal the structural basis of fiber formation. Comparison of chaperone bound Caf1 subunit with the subunit in the fiber reveals a novel type of conformational change involving the entire hydrophobic core of the protein. The observed conformational change suggests that the chaperone traps a high-energy folding intermediate of Caf1. A model is proposed in which release of the subunit allows folding to be completed, driving fiber formation.

About this Structure

1P5V is a Protein complex structure of sequences from Yersinia pestis. Full crystallographic information is available from OCA.

Reference

Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation., Zavialov AV, Berglund J, Pudney AF, Fooks LJ, Ibrahim TM, MacIntyre S, Knight SD, Cell. 2003 May 30;113(5):587-96. PMID:12787500

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