1p7d
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1p7d.gif|left|200px]] | + | [[Image:1p7d.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure of the Lambda Integrase (residues 75-356) bound to DNA''' | + | {{Structure |
| + | |PDB= 1p7d |SIZE=350|CAPTION= <scene name='initialview01'>1p7d</scene>, resolution 2.95Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= INT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the Lambda Integrase (residues 75-356) bound to DNA''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1P7D is a [ | + | 1P7D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P7D OCA]. |
==Reference== | ==Reference== | ||
| - | A conformational switch controls the DNA cleavage activity of lambda integrase., Aihara H, Kwon HJ, Nunes-Duby SE, Landy A, Ellenberger T, Mol Cell. 2003 Jul;12(1):187-98. PMID:[http:// | + | A conformational switch controls the DNA cleavage activity of lambda integrase., Aihara H, Kwon HJ, Nunes-Duby SE, Landy A, Ellenberger T, Mol Cell. 2003 Jul;12(1):187-98. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12887904 12887904] |
[[Category: Enterobacteria phage lambda]] | [[Category: Enterobacteria phage lambda]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 20: | Line 29: | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:20:49 2008'' |
Revision as of 11:20, 20 March 2008
| |||||||
| , resolution 2.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | INT (Enterobacteria phage lambda) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Lambda Integrase (residues 75-356) bound to DNA
Overview
The bacteriophage lambda integrase protein (lambda Int) belongs to a family of tyrosine recombinases that catalyze DNA rearrangements. We have determined a crystal structure of lambda Int complexed with a cleaved DNA substrate through a covalent phosphotyrosine bond. In comparison to an earlier unliganded structure, we observe a drastic conformational change in DNA-bound lambda Int that brings Tyr342 into the active site for cleavage of the DNA in cis. A flexible linker connects the central and the catalytic domains, allowing the protein to encircle the DNA. Binding specificity is achieved through direct interactions with the DNA and indirect readout of the flexibility of the att site. The conformational switch that activates lambda Int for DNA cleavage exposes the C-terminal 8 residues for interactions with a neighboring Int molecule. The protein interactions mediated by lambda Int's C-terminal tail offer a mechanism for the allosteric control of cleavage activity in higher order lambda Int complexes.
About this Structure
1P7D is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.
Reference
A conformational switch controls the DNA cleavage activity of lambda integrase., Aihara H, Kwon HJ, Nunes-Duby SE, Landy A, Ellenberger T, Mol Cell. 2003 Jul;12(1):187-98. PMID:12887904
Page seeded by OCA on Thu Mar 20 13:20:49 2008
