This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2i1t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2i1t" [edit=sysop:move=sysop])
Line 1: Line 1:
-
[[Image:2i1t.png|left|200px]]
+
==Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels==
 +
<StructureSection load='2i1t' size='340' side='right' caption='[[2i1t]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2i1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chilobrachys_guangxiensis Chilobrachys guangxiensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I1T FirstGlance]. <br>
 +
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [http://www.ebi.ac.uk/pdbsum/2i1t PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
We have isolated a cardiotoxin, denoted jingzhaotoxin-III (JZTX-III), from the venom of the Chinese spider Chilobrachys jingzhao. The toxin contains 36 residues stabilized by three intracellular disulfide bridges (I-IV, II-V, and III-VI), assigned by a chemical strategy of partial reduction and sequence analysis. Cloned and sequenced using 3'-rapid amplification of cDNA ends and 5'-rapid amplification of cDNA ends, the full-length cDNA encoded a 63-residue precursor of JZTX-III. Different from other spider peptides, it contains an uncommon endoproteolytic site (-X-Ser-) anterior to mature protein and the intervening regions of 5 residues, which is the smallest in spider toxin cDNAs identified to date. Under whole cell recording, JZTX-III showed no effects on voltage-gated sodium channels (VGSCs) or calcium channels in dorsal root ganglion neurons, whereas it significantly inhibited tetrodotoxin-resistant VGSCs with an IC(50) value of 0.38 microm in rat cardiac myocytes. Different from scorpion beta-toxins, it caused a 10-mV depolarizing shift in the channel activation threshold. The binding site for JZTX-III on VGSCs is further suggested to be site 4 with a simple competitive assay, which at 10 microm eliminated the slowing currents induced by Buthus martensi Karsch I (BMK-I, scorpion alpha-like toxin) completely. JZTX-III shows higher selectivity for VGSC isoforms than other spider toxins affecting VGSCs, and the toxin hopefully represents an important ligand for discriminating cardiac VGSC subtype.
-
{{STRUCTURE_2i1t| PDB=2i1t | SCENE= }}
+
Jingzhaotoxin-III, a novel spider toxin inhibiting activation of voltage-gated sodium channel in rat cardiac myocytes.,Xiao Y, Tang J, Yang Y, Wang M, Hu W, Xie J, Zeng X, Liang S J Biol Chem. 2004 Jun 18;279(25):26220-6. Epub 2004 Apr 14. PMID:15084603<ref>PMID:15084603</ref>
-
===Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_15084603}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
[[2i1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chilobrachys_guangxiensis Chilobrachys guangxiensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA].
+
</StructureSection>
[[Category: Chilobrachys guangxiensis]]
[[Category: Chilobrachys guangxiensis]]
-
[[Category: Liang, S.]]
+
[[Category: Liang, S]]
-
[[Category: Liao, Z.]]
+
[[Category: Liao, Z]]
-
[[Category: Peng, K.]]
+
[[Category: Peng, K]]
[[Category: Cardiac myocyte]]
[[Category: Cardiac myocyte]]
[[Category: Jingzhaotoxin-iii]]
[[Category: Jingzhaotoxin-iii]]

Revision as of 06:31, 22 December 2014

Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2i1t"
Personal tools