1pgy
From Proteopedia
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| - | [[Image:1pgy.jpg|left|200px]] | + | [[Image:1pgy.jpg|left|200px]] |
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| - | '''Solution structure of the UBA domain in Saccharomyces cerevisiae protein, Swa2p''' | + | {{Structure |
| + | |PDB= 1pgy |SIZE=350|CAPTION= <scene name='initialview01'>1pgy</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= SWA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of the UBA domain in Saccharomyces cerevisiae protein, Swa2p''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1PGY is a [ | + | 1PGY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PGY OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of the ubiquitin-binding domain in Swa2p from Saccharomyces cerevisiae., Chim N, Gall WE, Xiao J, Harris MP, Graham TR, Krezel AM, Proteins. 2004 Mar 1;54(4):784-93. PMID:[http:// | + | Solution structure of the ubiquitin-binding domain in Swa2p from Saccharomyces cerevisiae., Chim N, Gall WE, Xiao J, Harris MP, Graham TR, Krezel AM, Proteins. 2004 Mar 1;54(4):784-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14997574 14997574] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ubiquitin-associated domain]] | [[Category: ubiquitin-associated domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:24:15 2008'' |
Revision as of 11:24, 20 March 2008
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| Gene: | SWA2 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the UBA domain in Saccharomyces cerevisiae protein, Swa2p
Overview
The SWA2/AUX1 gene has been proposed to encode the Saccharomyces cerevisiae ortholog of mammalian auxilin. Swa2p is required for clathrin assembly/dissassembly in vivo, thereby implicating it in intracellular protein and lipid trafficking. While investigating the 287-residue N-terminal region of Swa2p, we found a single stably folded domain between residues 140 and 180. Using binding assays and structural analysis, we established this to be a ubiquitin-associated (UBA) domain, unidentified by bioinformatics of the yeast genome. We determined the solution structure of this Swa2p domain and found a characteristic three-helix UBA fold. Comparisons of structures of known UBA folds reveal that the position of the third helix is quite variable. This helix in Swa2p UBA contains a bulkier tyrosine in place of smaller residues found in other UBAs and cannot pack as close to the second helix. The molecular surface of Swa2p UBA has a mostly negative potential, with a single hydrophobic surface patch found also in the UBA domains of human protein, HHR23A. The presence of a UBA domain implicates Swa2p in novel roles involving ubiquitin and ubiquitinated substrates. We propose that Swa2p is a multifunctional protein capable of recognizing several proteins through its protein-protein recognition domains.
About this Structure
1PGY is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Solution structure of the ubiquitin-binding domain in Swa2p from Saccharomyces cerevisiae., Chim N, Gall WE, Xiao J, Harris MP, Graham TR, Krezel AM, Proteins. 2004 Mar 1;54(4):784-93. PMID:14997574
Page seeded by OCA on Thu Mar 20 13:24:15 2008
