1bfe
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Modular PDZ domains, found in many cell junction-associated proteins, mediate the clustering of membrane ion channels by binding to their, C-terminus. The X-ray crystallographic structures of the third PDZ domain, from the synaptic protein PSD-95 in complex with and in the absence of its, peptide ligand have been determined at 1.8 angstroms and 2.3 angstroms, resolution, respectively. The structures reveal that a four-residue, C-terminal stretch (X-Thr/Ser-X-Val-COO(-)) engages the PDZ domain through, antiparallel main chain interactions with a beta sheet of the domain., Recognition of the terminal carboxylate group of the peptide is conferred, by a cradle of main chain amides provided by a Gly-Leu-Gly-Phe loop as, well as by an arginine side chain. Specific side chain interactions and a, . | + | Modular PDZ domains, found in many cell junction-associated proteins, mediate the clustering of membrane ion channels by binding to their, C-terminus. The X-ray crystallographic structures of the third PDZ domain, from the synaptic protein PSD-95 in complex with and in the absence of its, peptide ligand have been determined at 1.8 angstroms and 2.3 angstroms, resolution, respectively. The structures reveal that a four-residue, C-terminal stretch (X-Thr/Ser-X-Val-COO(-)) engages the PDZ domain through, antiparallel main chain interactions with a beta sheet of the domain., Recognition of the terminal carboxylate group of the peptide is conferred, by a cradle of main chain amides provided by a Gly-Leu-Gly-Phe loop as, well as by an arginine side chain. Specific side chain interactions and a, prominent hydrophobic pocket explain the selective recognition of the, C-terminal consensus sequence. |
==About this Structure== | ==About this Structure== | ||
| - | 1BFE is a | + | 1BFE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Structure known Active Site: PEP. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BFE OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein localization]] | [[Category: protein localization]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:12:56 2007'' |
Revision as of 12:07, 5 November 2007
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THE THIRD PDZ DOMAIN FROM THE SYNAPTIC PROTEIN PSD-95
Overview
Modular PDZ domains, found in many cell junction-associated proteins, mediate the clustering of membrane ion channels by binding to their, C-terminus. The X-ray crystallographic structures of the third PDZ domain, from the synaptic protein PSD-95 in complex with and in the absence of its, peptide ligand have been determined at 1.8 angstroms and 2.3 angstroms, resolution, respectively. The structures reveal that a four-residue, C-terminal stretch (X-Thr/Ser-X-Val-COO(-)) engages the PDZ domain through, antiparallel main chain interactions with a beta sheet of the domain., Recognition of the terminal carboxylate group of the peptide is conferred, by a cradle of main chain amides provided by a Gly-Leu-Gly-Phe loop as, well as by an arginine side chain. Specific side chain interactions and a, prominent hydrophobic pocket explain the selective recognition of the, C-terminal consensus sequence.
About this Structure
1BFE is a Single protein structure of sequence from Rattus norvegicus. Structure known Active Site: PEP. Full crystallographic information is available from OCA.
Reference
Crystal structures of a complexed and peptide-free membrane protein-binding domain: molecular basis of peptide recognition by PDZ., Doyle DA, Lee A, Lewis J, Kim E, Sheng M, MacKinnon R, Cell. 1996 Jun 28;85(7):1067-76. PMID:8674113
Page seeded by OCA on Mon Nov 5 14:12:56 2007
