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1pop
From Proteopedia
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| - | [[Image:1pop.gif|left|200px]] | + | [[Image:1pop.gif|left|200px]] |
| - | + | ||
| - | '''X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX''' | + | {{Structure |
| + | |PDB= 1pop |SIZE=350|CAPTION= <scene name='initialview01'>1pop</scene>, resolution 2.1Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene> and <scene name='pdbligand=MOH:METHANOL'>MOH</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Papain Papain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.2 3.4.22.2] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1POP is a [ | + | 1POP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1POP OCA]. |
==Reference== | ==Reference== | ||
| - | X-ray crystallographic structure of a papain-leupeptin complex., Schroder E, Phillips C, Garman E, Harlos K, Crawford C, FEBS Lett. 1993 Jan 2;315(1):38-42. PMID:[http:// | + | X-ray crystallographic structure of a papain-leupeptin complex., Schroder E, Phillips C, Garman E, Harlos K, Crawford C, FEBS Lett. 1993 Jan 2;315(1):38-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8416808 8416808] |
[[Category: Papain]] | [[Category: Papain]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: hydrolase(thiol protease)]] | [[Category: hydrolase(thiol protease)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:27:01 2008'' |
Revision as of 11:27, 20 March 2008
| |||||||
| , resolution 2.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Papain, with EC number 3.4.22.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX
Overview
The three-dimensional structure of the papain-leupeptin complex has been determined by X-ray crystallography to a resolution of 2.1 A (overall R-factor = 19.8%). The structure indicates that: (i) leupeptin contacts the S subsites of the papain active site and not the S' subsites; (ii) the 'carbonyl' carbon atom of the inhibitor is covalently bound by the Cys-25 sulphur atom of papain and is tetrahedrally coordinated; (iii) the 'carbonyl' oxygen atom of the inhibitor faces the oxyanion hole and makes hydrogen bond contacts with Gln-19 and Cys-25.
About this Structure
1POP is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
X-ray crystallographic structure of a papain-leupeptin complex., Schroder E, Phillips C, Garman E, Harlos K, Crawford C, FEBS Lett. 1993 Jan 2;315(1):38-42. PMID:8416808
Page seeded by OCA on Thu Mar 20 13:27:01 2008
