Clegg Gal4 sandbox

From Proteopedia

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spinqualitylabelsall models PDB ID 1d66 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
1d66, resolution 2.70Å ()
Ligands:
Structural annotation: Resources: CATH : 1D66B01 InterPro : Ipr007219, Ipr005600, Ipr001138, Ipr002409 Pfam : PF00172, PF03902 SCOP : d1d66a2, d1d66a1, d1d66b1, d1d66b2 UniProt : P04386
Functional annotation: Cellular component: nucleus Biological process: carbohydrate metabolic process positive regulation of transcription by galactose transcription galactose metabolic process regulation of transcription, DNA-dependent Molecular function: transcription factor activity DNA binding metal ion binding transcription activator activity zinc ion binding
Evolutionary conservation: Toggle Conservation Colors: Rows = identical sequences: Further Information: Caveats, Explanation, Complete Results at ConSurfDB
Resources:	FirstGlance, OCA, RCSB, PDBsum
Coordinates:	save as pdb, mmCIF, xml

DNA RECOGNITION BY GAL4: STRUCTURE OF A PROTEIN/DNA COMPLEX

{{A specific DNA complex of the 65-residue, N-terminal fragment of the yeast transcriptional activator, GAL4, has been analysed at 2.7 A resolution by X-ray crystallography. The protein binds as a dimer to a symmetrical 17-base-pair sequence. A small, Zn(2+)-containing domain recognizes a conserved CCG triplet at each end of the site through direct contacts with the major groove. A short coiled-coil dimerization element imposes 2-fold symmetry. A segment of extended polypeptide chain links the metal-binding module to the dimerization element and specifies the length of the site. The relatively open structure of the complex would allow another protein to bind coordinately with GAL4.}}

About this Structure

1d66 is a 4 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

See Also

• Gal4
• Hydrogen in macromolecular models
• User:Eric Martz/Introduction to Structural Bioinformatics I
• User:Eric Martz/Introduction to Structural Bioinformatics I%2C 2013
• User:Wayne Decatur/Biochem642 Molecular Visualization 2010 Fall Sessions
• User:Wayne Decatur/Biochem642 Molecular Visualization Sessions

Reference

• Marmorstein R, Carey M, Ptashne M, Harrison SC. DNA recognition by GAL4: structure of a protein-DNA complex. Nature. 1992 Apr 2;356(6368):408-14. PMID:1557122 doi:http://dx.doi.org/10.1038/356408a0