1puo
From Proteopedia
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| - | [[Image:1puo.gif|left|200px]] | + | [[Image:1puo.gif|left|200px]] |
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| - | '''Crystal structure of Fel d 1- the major cat allergen''' | + | {{Structure |
| + | |PDB= 1puo |SIZE=350|CAPTION= <scene name='initialview01'>1puo</scene>, resolution 1.85Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= CH2, CH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9685 Felis catus]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of Fel d 1- the major cat allergen''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1PUO is a [ | + | 1PUO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Felis_catus Felis catus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PUO OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of the major cat allergen Fel d 1, a member of the secretoglobin family., Kaiser L, Gronlund H, Sandalova T, Ljunggren HG, van Hage-Hamsten M, Achour A, Schneider G, J Biol Chem. 2003 Sep 26;278(39):37730-5. Epub 2003 Jul 8. PMID:[http:// | + | The crystal structure of the major cat allergen Fel d 1, a member of the secretoglobin family., Kaiser L, Gronlund H, Sandalova T, Ljunggren HG, van Hage-Hamsten M, Achour A, Schneider G, J Biol Chem. 2003 Sep 26;278(39):37730-5. Epub 2003 Jul 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12851385 12851385] |
[[Category: Felis catus]] | [[Category: Felis catus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: uteroglobin]] | [[Category: uteroglobin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:29:12 2008'' |
Revision as of 11:29, 20 March 2008
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| , resolution 1.85Å | |||||||
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| Ligands: | |||||||
| Gene: | CH2, CH1 (Felis catus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Fel d 1- the major cat allergen
Overview
The domestic cat (Felis domesticus) is one of the most important causes of allergic asthma worldwide. The dominating cat allergen, Fel d 1, is composed of two heterodimers. Recently, it has been shown that recombinant Fel d 1, consisting of chain 2 and chain 1 fused together without additional linker, has immunological properties indistinguishable from the natural heterodimeric protein. Herein, we report the crystal structure of recombinant monomeric Fel d 1 at 1.85-A resolution, determined by multi-wavelength anomalous diffraction using selenomethionine substituted protein. Fel d 1 is an all-helical protein and consists of eight helices. The two halves of the recombinant Fel d 1 molecule, corresponding to the wild-type Fel d 1 chains, are very similar in three-dimensional structure, despite the lack of significant sequence identity. The structure of the Fel d 1 presents a striking similarity to that of uteroglobin, a steroid-inducible cytokine-like molecule with anti-inflammatory and immunomodulatory properties. An internal, asymmetric cavity is formed in the Fel d 1 that could bind an endogenous ligand. The distribution of residues lining this cavity suggests that such a ligand must be amphipathic. The structure of Fel d 1 displays the localization of three previously defined Fel d 1 IgE epitopes on the surface of the protein. The three-dimensional structure provides a framework for rational design of hypoallergenic mutants aimed for treatment of cat allergy.
About this Structure
1PUO is a Single protein structure of sequence from Felis catus. Full crystallographic information is available from OCA.
Reference
The crystal structure of the major cat allergen Fel d 1, a member of the secretoglobin family., Kaiser L, Gronlund H, Sandalova T, Ljunggren HG, van Hage-Hamsten M, Achour A, Schneider G, J Biol Chem. 2003 Sep 26;278(39):37730-5. Epub 2003 Jul 8. PMID:12851385
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