1pxd

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[[Image:1pxd.gif|left|200px]]<br /><applet load="1pxd" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1pxd.gif|left|200px]]
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caption="1pxd, resolution 1.8&Aring;" />
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'''Crystal structure of the complex of jacalin with meso-tetrasulphonatophenylporphyrin.'''<br />
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{{Structure
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|PDB= 1pxd |SIZE=350|CAPTION= <scene name='initialview01'>1pxd</scene>, resolution 1.8&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SFP:5,10,15,20-TETRAKIS(4-SULPFONATOPHENYL)-21H,23H-PORPHINE'>SFP</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal structure of the complex of jacalin with meso-tetrasulphonatophenylporphyrin.'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1PXD is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer] with <scene name='pdbligand=SFP:'>SFP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PXD OCA].
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1PXD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PXD OCA].
==Reference==
==Reference==
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Porphyrin binding to jacalin is facilitated by the inherent plasticity of the carbohydrate-binding site: novel mode of lectin-ligand interaction., Goel M, Anuradha P, Kaur KJ, Maiya BG, Swamy MJ, Salunke DM, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):281-8. Epub 2004, Jan 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14747704 14747704]
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Porphyrin binding to jacalin is facilitated by the inherent plasticity of the carbohydrate-binding site: novel mode of lectin-ligand interaction., Goel M, Anuradha P, Kaur KJ, Maiya BG, Swamy MJ, Salunke DM, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):281-8. Epub 2004, Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14747704 14747704]
[[Category: Artocarpus integer]]
[[Category: Artocarpus integer]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: porphyrin]]
[[Category: porphyrin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:30:11 2008''

Revision as of 11:30, 20 March 2008

Image:1pxd.gif


PDB ID 1pxd

Drag the structure with the mouse to rotate
, resolution 1.8Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



Crystal structure of the complex of jacalin with meso-tetrasulphonatophenylporphyrin.


Overview

The crystal structure of the complex of meso-tetrasulfonatophenylporphyrin (H(2)TPPS) with jack fruit (Artocarpus integriflora) agglutinin (jacalin) has been determined at 1.8 A resolution. A porphyrin pair is sandwiched between two symmetry-related jacalin monomers in the crystal, leading to a cross-linking network of protein molecules. Apart from the stacking interactions, H(2)TPPS also forms hydrogen bonds, some involving water bridges, with jacalin at the carbohydrate-binding site. The residues that are involved in rendering galactopyranoside specificity to jacalin undergo conformational adjustments in order to accommodate the H(2)TPPS molecule. The water molecules at the carbohydrate-binding site of jacalin cement the jacalin-porphyrin interactions, optimizing their complementarity. Interactions of porphyrin with jacalin are relatively weak compared with those observed between galactopyranoside and jacalin, perhaps because the former largely involves water-mediated hydrogen bonds. While H(2)TPPS binds to jacalin at the carbohydrate-binding site as in the case of ConA, its mode of interaction with jacalin is very different. H(2)TPPS does not enter the carbohydrate-binding cavity of jacalin. Instead, it sits over the binding site. While the porphyrin binding is mediated by replicating the hydrogen-bonding network of mannopyranoside through the sulfonate atoms in the case of ConA, the plasticity associated with the carbohydrate-binding site accommodates the pluripotent porphyrin molecule in the case of jacalin through an entirely different set of interactions.

About this Structure

1PXD is a Protein complex structure of sequences from Artocarpus integer. Full crystallographic information is available from OCA.

Reference

Porphyrin binding to jacalin is facilitated by the inherent plasticity of the carbohydrate-binding site: novel mode of lectin-ligand interaction., Goel M, Anuradha P, Kaur KJ, Maiya BG, Swamy MJ, Salunke DM, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):281-8. Epub 2004, Jan 23. PMID:14747704

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