1py0

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[[Image:1py0.gif|left|200px]]<br /><applet load="1py0" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1py0.gif|left|200px]]
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caption="1py0, resolution 2.00&Aring;" />
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'''Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe'''<br />
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{{Structure
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|PDB= 1py0 |SIZE=350|CAPTION= <scene name='initialview01'>1py0</scene>, resolution 2.00&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=Y1:YTTRIUM+ION'>Y1</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=YMA:7,10,13-TRI(CARBOXYMETHYL)-5,15-DIOXO-4,7,10,13,16-PENTAAZA-1,19-DITHIANONADECANE'>YMA</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1PY0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=Y1:'>Y1</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=YMA:'>YMA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PY0 OCA].
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1PY0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PY0 OCA].
==Reference==
==Reference==
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A caged lanthanide complex as a paramagnetic shift agent for protein NMR., Prudencio M, Rohovec J, Peters JA, Tocheva E, Boulanger MJ, Murphy ME, Hupkes HJ, Kosters W, Impagliazzo A, Ubbink M, Chemistry. 2004 Jul 5;10(13):3252-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15224334 15224334]
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A caged lanthanide complex as a paramagnetic shift agent for protein NMR., Prudencio M, Rohovec J, Peters JA, Tocheva E, Boulanger MJ, Murphy ME, Hupkes HJ, Kosters W, Impagliazzo A, Ubbink M, Chemistry. 2004 Jul 5;10(13):3252-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15224334 15224334]
[[Category: Alcaligenes faecalis]]
[[Category: Alcaligenes faecalis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: nmr probe]]
[[Category: nmr probe]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:30:25 2008''

Revision as of 11:30, 20 March 2008

Image:1py0.gif


PDB ID 1py0

Drag the structure with the mouse to rotate
, resolution 2.00Å
Ligands: , , and
Coordinates: save as pdb, mmCIF, xml



Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe


Overview

A lanthanide complex, named CLaNP (caged lanthanide NMR probe) has been developed for the characterisation of proteins by paramagnetic NMR spectroscopy. The probe consists of a lanthanide chelated by a derivative of DTPA (diethylenetriaminepentaacetic acid) with two thiol reactive functional groups. The CLaNP molecule is attached to a protein by two engineered, surface-exposed, Cys residues in a bidentate manner. This drastically limits the dynamics of the metal relative to the protein and enables measurements of pseudocontact shifts. NMR spectroscopy experiments on a diamagnetic control and the crystal structure of the probe-protein complex demonstrate that the protein structure is not affected by probe attachment. The probe is able to induce pseudocontact shifts to at least 40 A from the metal and causes residual dipolar couplings due to alignment at a high magnetic field. The molecule exists in several isomeric forms with different paramagnetic tensors; this provides a fast way to obtain long-range distance restraints.

About this Structure

1PY0 is a Single protein structure of sequence from Alcaligenes faecalis. Full crystallographic information is available from OCA.

Reference

A caged lanthanide complex as a paramagnetic shift agent for protein NMR., Prudencio M, Rohovec J, Peters JA, Tocheva E, Boulanger MJ, Murphy ME, Hupkes HJ, Kosters W, Impagliazzo A, Ubbink M, Chemistry. 2004 Jul 5;10(13):3252-60. PMID:15224334

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