1q0v
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1q0v.jpg|left|200px]] | + | [[Image:1q0v.jpg|left|200px]] |
| - | + | ||
| - | '''Solution Structure of Tandem UIMs of Vps27''' | + | {{Structure |
| + | |PDB= 1q0v |SIZE=350|CAPTION= <scene name='initialview01'>1q0v</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= vps27 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Solution Structure of Tandem UIMs of Vps27''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1Q0V is a [ | + | 1Q0V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q0V OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of Vps27 UIM-ubiquitin complex important for endosomal sorting and receptor downregulation., Swanson KA, Kang RS, Stamenova SD, Hicke L, Radhakrishnan I, EMBO J. 2003 Sep 15;22(18):4597-606. PMID:[http:// | + | Solution structure of Vps27 UIM-ubiquitin complex important for endosomal sorting and receptor downregulation., Swanson KA, Kang RS, Stamenova SD, Hicke L, Radhakrishnan I, EMBO J. 2003 Sep 15;22(18):4597-606. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12970172 12970172] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 18: | Line 27: | ||
[[Category: Stamenova, S D.]] | [[Category: Stamenova, S D.]] | ||
[[Category: Swanson, K A.]] | [[Category: Swanson, K A.]] | ||
| - | [[Category: non-interacting alpha- | + | [[Category: non-interacting alpha-helice]] |
[[Category: stable]] | [[Category: stable]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:31:32 2008'' |
Revision as of 11:31, 20 March 2008
| |||||||
| Gene: | vps27 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of Tandem UIMs of Vps27
Overview
Monoubiquitylation is a well-characterized signal for the internalization and sorting of integral membrane proteins to distinct cellular organelles. Recognition and transmission of monoubiquitin signals is mediated by a variety of ubiquitin-binding motifs such as UIM, UBA, UEV, VHS and CUE in endocytic proteins. The yeast Vps27 protein requires two UIMs for efficient interactions with ubiquitin and for sorting cargo into multivesicular bodies. Here we show that the individual UIMs of Vps27 exist as autonomously folded alpha-helices that bind ubiquitin independently, non-cooperatively and with modest affinity. The Vps27 N-terminal UIM engages the Leu8-Ile44-Val70 hydrophobic patch of ubiquitin through a helical surface conserved in UIMs of diverse proteins, including that of the S5a proteasomal regulatory subunit. The Leu8-Ile44-Val70 ubiquitin surface is also the site of interaction for CUE and UBA domains in endocytic proteins, consistent with the view that ubiquitin-binding endocytic proteins act serially on the same monoubiquitylated cargo during transport from cell surface to the lysosome.
About this Structure
1Q0V is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Solution structure of Vps27 UIM-ubiquitin complex important for endosomal sorting and receptor downregulation., Swanson KA, Kang RS, Stamenova SD, Hicke L, Radhakrishnan I, EMBO J. 2003 Sep 15;22(18):4597-606. PMID:12970172
Page seeded by OCA on Thu Mar 20 13:31:32 2008
