1q1j
From Proteopedia
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| - | [[Image:1q1j.gif|left|200px]] | + | [[Image:1q1j.gif|left|200px]] |
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| - | '''Crystal Structure Analysis of anti-HIV-1 Fab 447-52D in complex with V3 peptide''' | + | {{Structure |
| + | |PDB= 1q1j |SIZE=350|CAPTION= <scene name='initialview01'>1q1j</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Crystal Structure Analysis of anti-HIV-1 Fab 447-52D in complex with V3 peptide''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Q1J is a [ | + | 1Q1J is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q1J OCA]. |
==Reference== | ==Reference== | ||
| - | Structural rationale for the broad neutralization of HIV-1 by human monoclonal antibody 447-52D., Stanfield RL, Gorny MK, Williams C, Zolla-Pazner S, Wilson IA, Structure. 2004 Feb;12(2):193-204. PMID:[http:// | + | Structural rationale for the broad neutralization of HIV-1 by human monoclonal antibody 447-52D., Stanfield RL, Gorny MK, Williams C, Zolla-Pazner S, Wilson IA, Structure. 2004 Feb;12(2):193-204. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14962380 14962380] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: v3 loop]] | [[Category: v3 loop]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:31:46 2008'' |
Revision as of 11:31, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure Analysis of anti-HIV-1 Fab 447-52D in complex with V3 peptide
Overview
447-52D is a human monoclonal antibody isolated from a heterohybridoma derived from an HIV-1-infected individual. This antibody recognizes the hypervariable gp120 V3 loop, and neutralizes both X4 and R5 primary isolates, making it one of the most effective anti-V3 antibodies characterized to date. The crystal structure of the 447-52D Fab in complex with a 16-mer V3 peptide at 2.5 A resolution reveals that the peptide beta hairpin forms a three-stranded mixed beta sheet with complementarity determining region (CDR) H3, with most of the V3 side chains exposed to solvent. Sequence specificity is conferred through interaction of the type-II turn (residues GPGR) at the apex of the V3 hairpin with the base of CDR H3. This novel mode of peptide-antibody recognition enables the antibody to bind to many different V3 sequences where only the GPxR core epitope is absolutely required.
About this Structure
1Q1J is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural rationale for the broad neutralization of HIV-1 by human monoclonal antibody 447-52D., Stanfield RL, Gorny MK, Williams C, Zolla-Pazner S, Wilson IA, Structure. 2004 Feb;12(2):193-204. PMID:14962380
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