1q35
From Proteopedia
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| - | [[Image:1q35.gif|left|200px]] | + | [[Image:1q35.gif|left|200px]] |
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| - | '''Crystal Structure of Pasteurella haemolytica Apo Ferric ion-Binding Protein A''' | + | {{Structure |
| + | |PDB= 1q35 |SIZE=350|CAPTION= <scene name='initialview01'>1q35</scene>, resolution 1.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> and <scene name='pdbligand=FMT:FORMIC ACID'>FMT</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= fbpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=75985 Mannheimia haemolytica]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Pasteurella haemolytica Apo Ferric ion-Binding Protein A''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Q35 is a [ | + | 1Q35 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mannheimia_haemolytica Mannheimia haemolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q35 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of Pasteurella haemolytica ferric ion-binding protein A reveals a novel class of bacterial iron-binding proteins., Shouldice SR, Dougan DR, Williams PA, Skene RJ, Snell G, Scheibe D, Kirby S, Hosfield DJ, McRee DE, Schryvers AB, Tari LW, J Biol Chem. 2003 Oct 17;278(42):41093-8. Epub 2003 Jul 25. PMID:[http:// | + | Crystal structure of Pasteurella haemolytica ferric ion-binding protein A reveals a novel class of bacterial iron-binding proteins., Shouldice SR, Dougan DR, Williams PA, Skene RJ, Snell G, Scheibe D, Kirby S, Hosfield DJ, McRee DE, Schryvers AB, Tari LW, J Biol Chem. 2003 Oct 17;278(42):41093-8. Epub 2003 Jul 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12882966 12882966] |
[[Category: Mannheimia haemolytica]] | [[Category: Mannheimia haemolytica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: iron binding protein]] | [[Category: iron binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:32:21 2008'' |
Revision as of 11:32, 20 March 2008
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| , resolution 1.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | fbpA (Mannheimia haemolytica) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Pasteurella haemolytica Apo Ferric ion-Binding Protein A
Overview
Pasteurellosis caused by the Gram-negative pathogen Pasteurella haemolytica is a serious disease leading to death in cattle. To scavenge growth-limiting iron from the host, the pathogen utilizes the periplasmic ferric ion-binding protein A (PhFbpA) as a component of an ATP-binding cassette transport pathway. We report the 1.2-A structure of the iron-free (apo) form of PhFbpA, which is a member of the transferrin structural superfamily. The protein structure adopts a closed conformation, allowing us to reliably assign putative iron-coordinating residues. Based on our analysis, PhFbpA utilizes a unique constellation of binding site residues and anions to octahedrally coordinate an iron atom. A surprising finding in the structure is the presence of two formate anions on opposite sides of the iron-binding pocket. The formate ions tether the N- and C-terminal domains of the protein and stabilize the closed structure, also providing clues as to probable candidates for synergistic anions in the iron-loaded state. PhFbpA represents a new class of bacterial iron-binding proteins.
About this Structure
1Q35 is a Single protein structure of sequence from Mannheimia haemolytica. Full crystallographic information is available from OCA.
Reference
Crystal structure of Pasteurella haemolytica ferric ion-binding protein A reveals a novel class of bacterial iron-binding proteins., Shouldice SR, Dougan DR, Williams PA, Skene RJ, Snell G, Scheibe D, Kirby S, Hosfield DJ, McRee DE, Schryvers AB, Tari LW, J Biol Chem. 2003 Oct 17;278(42):41093-8. Epub 2003 Jul 25. PMID:12882966
Page seeded by OCA on Thu Mar 20 13:32:21 2008
